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Cell Competency Test Lab Report

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Cell Competency Test Lab Report
The experiment was separated into four stages, the first being preparation of cell competency. In this stage two vials were placed in ice baths, one vial containing 50 µL of E. coli and the other containing a CaCl2 solution. 630 µL of the CaCl2 solution was then transferred to the E. coli vial, using a sterile pipet. After tapping the tube to mix the solution, it was then returned to the ice bath to continue incubation for at least 10 minutes. The cell competency preparation was carried out by the instructor in this experiment. Stage two was the uptake of DNA by competent cells. This stage was carried out by five groups, three performing plasmid pUC18 and two groups performing plasmid lux. Each group labeled two Eppendorf tubes, one was labeled
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