Energy is extracted from ATP by the chemical reaction of hydrolysis. As mentioned earlier, hydrolysis is the reverse reaction of dehydration synthesis. When water is added to ATP, the bond between the gamma phosphate group and beta
phosphate group (shown left) is broken. This separates ATP into ADP and inorganic phosphate, releasing energy.
The energy that is released from the hydrolysis of ATP is used by transport proteins to power the pumping of different molecules across the membrane. Without this simple yet powerful chemical reaction, active transport could not occur. This would mean that many molecules would not be able to cross plasma membranes such as the cell membrane. Essential molecules would not be able to enter or exit the cell. As a result, normal biological processes could not occur and we would die. Active transport is a clear indicator that chemical reactions like hydrolysis are very important.