Ciprofloxacin Lab Report

Question: What antibiotics work best in preventing E. coli k12 from growing; amoxicillin, ampicillin, or ciprofloxacin?

Hypothesis: If ciprofloxacin, an antibiotic, is added to petri dishes covered in live bacteria and left to sit for four to six days in an incubator at 37 degrees Celsius, then the petri dishes containing ciprofloxacin will have the largest zone of inhibition out of all of the other antibiotics tested because research shows that ciprofloxacin in one of the strongest antibiotic currently being sold on the market. The chemical formulas for the three antibiotics that I am using are as follows:
Amoxicillin Chemical Formula: C16H19N3O5S
Ampicillin Chemical Formula: C16H19N3O4S
Ciprofloxacin Chemical Formula: C17H18FN3O3
Ampicillin
After introducing the bacteria to individual petri dishes, quickly place the lid back on to prevent any contamination.

2. Label the 18 Petri Dishes- Amoxicillin, Ampicillin, Ciprofloxacin, and Control. There should be four labels of each antibiotic and six controls. When doing the following steps, make sure you are by a Bunsen burner because the heat radiating will kill off any other bacteria in the air and prevent contamination of the experiment.
a. Keep the antibiotic disks refrigerated until ready for use.
b. After introducing the live cultures of bacteria to each of the 18 petri dishes, remove the antibiotics (amoxicillin, ampicillin, and ciprofloxacin) from the
Using a pair of clippers, remove individual disks of amoxicillin from the magazine and place them in the middle of each petri dish labeled ‘amoxicillin’.
d. Using a pair of clippers, remove individual disks of ampicillin from the magazine and place them in the middle of each petri dish labeled ‘ampicillin’.
e. Using a pair of clippers, remove individual disks of ciprofloxacin from the magazine and place them in the middle of each petri dish labeled ‘ciprofloxacin’.
f. Using a hole-puncher, punch out six disks of filter paper to use for your control.
g. Dip each of the six disks of filter paper in water, and place each individual disk in the middle of the petri dishes labeled “control”.
h. After placing each disk into individual petri dishes, quickly place the lid back on to prevent any contamination.

3. Place the petri dishes in an incubator for several days. [Remember to store the dishes upside down, so the bacterial growth remains undisturbed by any water droplets] -The incubator should be set to the ideal temperature of 37 Degrees Celsius.

4. Leave the bacteria to develop for 4 days, as this will give the cultures enough time to