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Five Second Rule

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Five Second Rule
A. Introduction/Purpose:
This researcher chose the five second rule to investigate if the five second rule is accurate or not. Knowing if the five second rule is accurate or not will help keep people healthy and prevent them from getting sick. The five second rule contributes to the field of study by whether the food dropped on the floor will grow bacteria before five seconds or only after five seconds.

B. Investigative Question:
Does picking up fallen food from the ground within five seconds prevent the contamination and growth of bacteria?

C. Background Research:
“See ‘The Five-Second Rule’”

D. Hypothesis:
If the food falls on the ground for less than 5 seconds then it will be contaminated and grow bacteria.

E. Experimental Design:
…show more content…
Materials
• 22 prepared nutrient agar in petri dishes • Timer
• Starburst candy (11) • 22 sterile swab
• ½ slice of Bologna- Oscar Myer (11) • Sterile gloves

G. Procedures
1) Prepare 4 petri dishes with nutrient agar.
2) Put on your sterile gloves
3) Pick up your first food (Starburst candy) and drop it on the ground.
4) Start the timer.
5) Pick up the Starburst candy from the ground after 3 seconds.
6) Swab Starburst candy with a sterile swab.
7) Open the top of the petri dish and take the swab in a zigzag pattern on the top of the nutrient agar.
8) Put the top of the petri dish back on and label type of food and time.
9) Change gloves
10) Repeat steps 3-8 except pick up the Starburst candy from the ground at 8 seconds.
11) Change gloves.
12) Pick up your second food (bologna) and drop it onto the ground.
13) Start the timer.
14) Pick up the bologna from the ground after 3 seconds.
15) Swab bologna with a sterile swab.
16) Open the top of the petri dish and take the swab in a zigzag pattern on the top of the nutrient agar.
17) Put the top of the petri dish back on and label type of food and time.
18) Change gloves.
19) Repeat steps 12-17 except pick up the bologna from the ground at 8 seconds.
20) Repeat all the steps above 4 more

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