cloning steps using plasmid vector
UNIVERSITY OF DAR ES SAALAM
COLLAGE OF NATURAL AND APPLIED SCIENCE
DEPARTMENT OF MOLECURAL BIOLOGYG AND BIOTECHNOLOGY
GENE CLONING .
NAME: KANENDA. Ally. O
REG #: 2013-04-02841
DEGREE PROGRAM: MOLECURAL BIOLOGY AND BIOTECHNOLOGY
COURSE INSTRUCTOR: Dr. ROSE MASALU
Gene cloning:
Gene cloning is the process of inserting a gene of importance into a recipient organism, usually a bacterium, in order to replicate that gene many times and mass-produce a useful gene product. The recipient organism is called the host, and the material used to introduce the gene, usually a plasmid or bacteriophage, is called the vector. Ideal vectors have high rates of replication. Gene cloning is an important procedure in genetic engineering that allows us to produce a large amount of a useful gene product: for instance, insulin can be made for diabetic individuals by cloning genes from pancreatic cells.(Wikipedia 15/11/2014)
Cloning vector:
A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.( Genome Dictionary. Retrieved 2012-10-18.)
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector. There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids. Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages (such as phage λ), cosmids, and bacterial artificial chromosomes (BACs). Some DNA however cannot be stably maintained in E. coli, for example very large DNA fragment, and other organisms such as yeast may be used. Cloning vectors in yeast include yeast artificial chromosomes (YACs).
Plasmid is an autonomously replicating circular extra-chromosomal DNA. They are the standard cloning vectors and the most commonly used.
COLLAGE OF NATURAL AND APPLIED SCIENCE
DEPARTMENT OF MOLECURAL BIOLOGYG AND BIOTECHNOLOGY
GENE CLONING .
NAME: KANENDA. Ally. O
REG #: 2013-04-02841
DEGREE PROGRAM: MOLECURAL BIOLOGY AND BIOTECHNOLOGY
COURSE INSTRUCTOR: Dr. ROSE MASALU
Gene cloning:
Gene cloning is the process of inserting a gene of importance into a recipient organism, usually a bacterium, in order to replicate that gene many times and mass-produce a useful gene product. The recipient organism is called the host, and the material used to introduce the gene, usually a plasmid or bacteriophage, is called the vector. Ideal vectors have high rates of replication. Gene cloning is an important procedure in genetic engineering that allows us to produce a large amount of a useful gene product: for instance, insulin can be made for diabetic individuals by cloning genes from pancreatic cells.(Wikipedia 15/11/2014)
Cloning vector:
A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.( Genome Dictionary. Retrieved 2012-10-18.)
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector. There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids. Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages (such as phage λ), cosmids, and bacterial artificial chromosomes (BACs). Some DNA however cannot be stably maintained in E. coli, for example very large DNA fragment, and other organisms such as yeast may be used. Cloning vectors in yeast include yeast artificial chromosomes (YACs).
Plasmid is an autonomously replicating circular extra-chromosomal DNA. They are the standard cloning vectors and the most commonly used.