Column and Thin Layer Chromatography
COLUMN AND THIN LAYER CHROMATOGRAPHY
Group 9
RAMOS, Sharina Joy; REYES Aina Marie; REYES Jallisa Maan;
RUBIO, John Michael; SABINO Patricia Anne; SANTOS, Carlos Rafael
ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components, the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment, the solid used by the students was silica gel. Chromatography is a method of separating mixtures and at the same time useful in the identification of compounds. It is based on both solubility and absorptivity. The students were able to extract, more or less, six (6) colors and was further broken down into three (3) colors specifically yellow, red and orange. Using a precoated TLC plate, the students then was able to observe and measure the Rf values of the different components of the siling labuyo through the use of the TLC plates with eluates applied and was then placed in the developing chamber. At the end of the experiment, the students were able to discover the different components of the siling labuyo, its purity and the Rf values of each components of the red siling labuyo.
INTRODUCTION The experiment was done to separate the colored components of siling labuyo using column chromatography; to determine the purity of the components using the thin layer chromatography; and to measure the Rf values of the colored components in thin layer chromatography. Chromatography is the most modern and sophisticated method of separating mixtures and identifications of compounds in a solution. The procedures rely on the adsorptivities of the components to be separated. There are two involved phases in chromatography namely, the stationary phase which is the substance fixed in place and the mobile phase which is the phase that moves in definite direction. The experiment focused on the column and thin layer
Group 9
RAMOS, Sharina Joy; REYES Aina Marie; REYES Jallisa Maan;
RUBIO, John Michael; SABINO Patricia Anne; SANTOS, Carlos Rafael
ABSTRACT To separate the colored components of siling labuyo and to determine the purity of the components, the students performed a column and thin or solid-liquid chromatography procedure. The solid may be almost any material that does not dissolve in liquid phase. But for this experiment, the solid used by the students was silica gel. Chromatography is a method of separating mixtures and at the same time useful in the identification of compounds. It is based on both solubility and absorptivity. The students were able to extract, more or less, six (6) colors and was further broken down into three (3) colors specifically yellow, red and orange. Using a precoated TLC plate, the students then was able to observe and measure the Rf values of the different components of the siling labuyo through the use of the TLC plates with eluates applied and was then placed in the developing chamber. At the end of the experiment, the students were able to discover the different components of the siling labuyo, its purity and the Rf values of each components of the red siling labuyo.
INTRODUCTION The experiment was done to separate the colored components of siling labuyo using column chromatography; to determine the purity of the components using the thin layer chromatography; and to measure the Rf values of the colored components in thin layer chromatography. Chromatography is the most modern and sophisticated method of separating mixtures and identifications of compounds in a solution. The procedures rely on the adsorptivities of the components to be separated. There are two involved phases in chromatography namely, the stationary phase which is the substance fixed in place and the mobile phase which is the phase that moves in definite direction. The experiment focused on the column and thin layer
References: From books: [1] Bayquen, A.V., Cruz, C.T., de Guia, R.M., Lampa, F.F., Pena, G.T., Sarile, A.S., and Torres, P.C. (2009) Organic Chemistry. 839 EDSA, South Triangle, Quezon City: C & E Publishing, Inc. From the internet (on-line): [2]http://chemwiki.ucdavis.edu/Analytical_Chemistry/Instrumental_Analysis/Chromatography