Liquid Chromatography Lab
Liquid Chromatography – Laboratory #18
Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes, and then we separate the flavorings and citric acids.
Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid. The mixture we are trying to split up is integrated into the mobile phase. When the mobile phase interacts with the solid phase, the components of the mixture are attracted to the solid phase in varying degrees. Components with higher levels of attraction for the mobile phase result in a faster speed of transport throughout the solid phase. Components with higher levels of attraction for the solid phase result in a slower speed of transport throughout the solid phase. These differing levels of attraction end up in separation of the mixture into component bands, which exit the system as distinct liquids.
[pic]
Liquid chromatography labs are composed of six main parts:
a. A separation column filled with a fine-grain solid.
b. A solvent (mobile phase) that moves through the separation column.
c.An injection system to transport the solvent to the separation column.
d. A pump to force the solvent through the separation column.
e. A detector to determine when the components exit the separation column.
f. A recorder
[pic]
Although in most chromatography labs the solid phase is polar and the mobile phase is nonpolar, we are using Reverse Phase Liquid Chromatography, where the mobile phase is polar and the solid phase is nonpolar.
When the mobile phase interacts with the solid phase, the polar parts of the mobile phase are attracted to each other, so they wash through the column quickly. The less polar components of the mobile phase are attracted
Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes, and then we separate the flavorings and citric acids.
Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid. The mixture we are trying to split up is integrated into the mobile phase. When the mobile phase interacts with the solid phase, the components of the mixture are attracted to the solid phase in varying degrees. Components with higher levels of attraction for the mobile phase result in a faster speed of transport throughout the solid phase. Components with higher levels of attraction for the solid phase result in a slower speed of transport throughout the solid phase. These differing levels of attraction end up in separation of the mixture into component bands, which exit the system as distinct liquids.
[pic]
Liquid chromatography labs are composed of six main parts:
a. A separation column filled with a fine-grain solid.
b. A solvent (mobile phase) that moves through the separation column.
c.An injection system to transport the solvent to the separation column.
d. A pump to force the solvent through the separation column.
e. A detector to determine when the components exit the separation column.
f. A recorder
[pic]
Although in most chromatography labs the solid phase is polar and the mobile phase is nonpolar, we are using Reverse Phase Liquid Chromatography, where the mobile phase is polar and the solid phase is nonpolar.
When the mobile phase interacts with the solid phase, the polar parts of the mobile phase are attracted to each other, so they wash through the column quickly. The less polar components of the mobile phase are attracted