paper chromatography
PAPER and COLUMN CHROMATOGRAPHY REPORT
1.
2. The unknown code is an amino acid, Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine
3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase, the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
4. Based on the nature of the mobile and stationary phases used in this experiment, the size of the amino acids is affected depending on the distance of the Rf. The smaller the Rf, the smaller it has on the size. The larger the Rf, the larger the size on the amino acid.
5. Based on the nature of the mobile and stationary phases used in this experiment the effect of the polarity of the side chain is that the distance will not rise up on the paper if it is polar. The paper is made of cellulose which is polar so the attraction to the paper will not let the amino acids go any further up the paper. When the amino acids rise up quickly it is known as a nonpolar.
6. Tryptophane is the largest amino acid. Also two larger amino acids are Alanine and Serine. The largest Rf is Tryptophane. The polarity is like the Serine but the Alanine is nonpolar.
7. The largest Rf is the Serine out of Serine, Lysine and Aspartic Acid. The serine is also a medium in size and rather close to the other amino acids.
8. There are many trends that are observed between the Rf and size and polarity of the amino acid chains. The Rf helps determine the polarity and the relative size of the experiment. The distance and greater amount of the Rf gives the size which is from smallest to largest. The higher the number the larger the amino acid is. The polarity is based on the absorption of the paper. The medium sized amino acids tend to be the most relevant to being polar. The further away the
1.
2. The unknown code is an amino acid, Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine
3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase, the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
4. Based on the nature of the mobile and stationary phases used in this experiment, the size of the amino acids is affected depending on the distance of the Rf. The smaller the Rf, the smaller it has on the size. The larger the Rf, the larger the size on the amino acid.
5. Based on the nature of the mobile and stationary phases used in this experiment the effect of the polarity of the side chain is that the distance will not rise up on the paper if it is polar. The paper is made of cellulose which is polar so the attraction to the paper will not let the amino acids go any further up the paper. When the amino acids rise up quickly it is known as a nonpolar.
6. Tryptophane is the largest amino acid. Also two larger amino acids are Alanine and Serine. The largest Rf is Tryptophane. The polarity is like the Serine but the Alanine is nonpolar.
7. The largest Rf is the Serine out of Serine, Lysine and Aspartic Acid. The serine is also a medium in size and rather close to the other amino acids.
8. There are many trends that are observed between the Rf and size and polarity of the amino acid chains. The Rf helps determine the polarity and the relative size of the experiment. The distance and greater amount of the Rf gives the size which is from smallest to largest. The higher the number the larger the amino acid is. The polarity is based on the absorption of the paper. The medium sized amino acids tend to be the most relevant to being polar. The further away the