Paperose Lab Activity
Paperose Lab- Introduction to Enzymes Ms. Kim/Honors Biology
Purpose
To Determine the rate of an enzyme reaction using a “modeled” enzyme and sugar, paperase and paperose.
Introduction
In this lab, your hands are the enzyme, paperase. This enzyme split the sugar, paperose, into subunit A and subunit B. You will split this molecule by ripping the paper model down the middle.
Materials
Paperose models, scissors, plastic bag, container (large plastic cup), stopwatch,
Procedure
1. Each member will cut out molecules of paperose from the paper that your teacher gives you.
2. Your group should have 50 molecules of white paperose.
3. Place all 50 molecules in a container.
4. One member will act as the enzyme (paperase), one member will be the timer, one member will be the announcer and one member will be the recorder and will write down the number of molecules ripped by each time interval on the data worksheets.
a. The person who acts as the enzyme must follow these rules:
i. when told to begin, grab one (1) paperose molecule from the container and rip it down the middle. ii. Place the pieces back into the container and grab another whole paperose molecule. iii. Do not look into the container. iv. If you grab a molecule that is already ripped, throw it back in and try again.
v. Count the number of molecules ripped aloud as you go. vi. Try to work at a consistent pace throughout.
b. The timer will tell the enzyme when to begin and will tell the announcer when they have reached each time interval (10 seconds, 30 seconds, 60 seconds, 120 seconds, 180 seconds, and 360 seconds). The time intervals are cumulative. The enzyme should keep ripping and counting for the entire 360 seconds. If the enzyme runs out of paperose molecules, none should be added to the container.
c. The announcer will tell the recorder how many paperose sugars the paperase enzyme has broken at each time interval. These numbers will be cumulative and should NOT start
Purpose
To Determine the rate of an enzyme reaction using a “modeled” enzyme and sugar, paperase and paperose.
Introduction
In this lab, your hands are the enzyme, paperase. This enzyme split the sugar, paperose, into subunit A and subunit B. You will split this molecule by ripping the paper model down the middle.
Materials
Paperose models, scissors, plastic bag, container (large plastic cup), stopwatch,
Procedure
1. Each member will cut out molecules of paperose from the paper that your teacher gives you.
2. Your group should have 50 molecules of white paperose.
3. Place all 50 molecules in a container.
4. One member will act as the enzyme (paperase), one member will be the timer, one member will be the announcer and one member will be the recorder and will write down the number of molecules ripped by each time interval on the data worksheets.
a. The person who acts as the enzyme must follow these rules:
i. when told to begin, grab one (1) paperose molecule from the container and rip it down the middle. ii. Place the pieces back into the container and grab another whole paperose molecule. iii. Do not look into the container. iv. If you grab a molecule that is already ripped, throw it back in and try again.
v. Count the number of molecules ripped aloud as you go. vi. Try to work at a consistent pace throughout.
b. The timer will tell the enzyme when to begin and will tell the announcer when they have reached each time interval (10 seconds, 30 seconds, 60 seconds, 120 seconds, 180 seconds, and 360 seconds). The time intervals are cumulative. The enzyme should keep ripping and counting for the entire 360 seconds. If the enzyme runs out of paperose molecules, none should be added to the container.
c. The announcer will tell the recorder how many paperose sugars the paperase enzyme has broken at each time interval. These numbers will be cumulative and should NOT start