Curcumin Case Study

For the treatment of cancer most of the drugs currently available but they have limited therapeutic potential because they are very toxic, expensive, highly inappropriate action in treating cancer, or having so many side effects like granulocytopenia, agranulocytosis, lymphocytopenia, alopecia, carcinogenicity or secondary cancer especially leukaemias and hyperuricaemia etc. Curcumin is one such natural herbal medicine, derived from the extraction of dried rhizome of turmeric or Curcumin longa, contain bioactive compounds with powerful therapeutic properties[1-3] therefore, it has been used for thousands of years in the orient as a healing agent for variety of illnesses like as a anti-inflammatory disease,[4] it help to increase antioxidant
al. curcumin capped AuNPs, and curcumin glycoside also used as a reducing and stabilizing agents, and conjugates shown long term colloidal stability. They described the possible mechanism of stabilization is due to chelation of gold with 1,3-diketone functionality of the curcumin. Further they evaluated the cytotoxic behaviour of curcumin glycoside-AuNPs conjugates. Sindhu and rajaram et. al. have synthesized the AuNPs-Curcumin conjugates in alkaline pH at room temperature without using reducing and stabilizing agent because curcumin shows reducing and stabilizing behavior. The non toxic nature of the AuNPs-Curcumin described through biocompatibility studies using human blood cells.[27] In order to improve bioavailability Poddar et. al. have also given a new protocol of AuNPs-Curcumin conjugates and evaluated antioxidant properties of curcumin, which they confirmed by the 2,2-diphenyl-l-picryl hydrazyl (DPPH) radical test. In their synthesis curcumin again act as reducing and capping agent, and help to stabilize gold for long time. Other from their experimental study, they performed a detailed quantum chemical calculation using density functional theory or DFT, and described the formation of a complex of curcumin with Au3+ ions in different conformational isomeric forms.[28] Suwarna Datar et. al. have reported the conjugation of curcumin with gold nanoparticle and they used PVP K-40 as a linker which form a hydrogen bonding with citrate
Conjugation of curcumin with polyvinlypyrrolidone(PVP) Tri-sodium citrate and curcumin both act as a capping and stabilizing agent. 100ml of 3.88mM tri-sodium citrate in water heated under reflux condenser and 20mg polyvinyl pyrrolidone in 30 ml water was prepared and added in 100ml of trisodium citrate solution and boiled the solution for 15min. the solution was cooled and For the conjugation of curcumin 50mg of curcumin dissolved in 25ml of acetone and this solution added to above solution and reaction mixture stirred continue for 3hr at 60ºC without reflux condenser for complete removal of acetone from the