Explain Why Curcumin Has Been Loaded Into Many Nanocarriers
Curcumin has been loaded into many nanocarriers, while it’s a meaningful attempt to load it into a typical protein capsid lumazine synthase. The highly symmetrical structure of AaLS-IC capsid can improve drug loading capacity and the biocompatibility of AaLS-IC capsid can improve the solubility of curcumin and protect it from environment. In addition, the simple and high-efficient process of curcumin loading and unnloading are also attractive. After a two-step reaction, the AaLS-IC-cur can be made and the yield of AaLS-IC-cur is 70%, it’s mainly depended on the reduction of the activated disulfide 5,5’-dithiobisnitrobenzoic acid (DTNB). It’s a high enough yield for curcumin loading.
4.2 The solubility of AaLS-IC-cur
Loading cur-SH into
AaLS-IC capsid can improve the poor solubility of free cur-SH in aqueous. Depending on a simple comparison of cur-SH and AaLS-IC-cur in PBS buffer with 0.8% ethanol, the solubility of AaLS-IC-cur is more than 30 times higher than cur-SH, that means loading of cur-SH into AaLS capsid can provide a better bioavailability.
4.3 The release of AaLS-IC-cur
About 60 % to 80 % of cur-SH can be free in a short time course when AaLS-IC-cur conjugate was treated with suitable reducing reagent (TCEP). While the hydrophobic property of cur-SH make more than 80 % free cur-SH cargos keep in hydrophobic cavity of capsid when cur-SH were saturated in aqueous solution. While the poor stability of cur-SH and AaLS-IC-cur in aqueous solution is a limitation for its further application.
4.2 The solubility of AaLS-IC-cur
Loading cur-SH into
AaLS-IC capsid can improve the poor solubility of free cur-SH in aqueous. Depending on a simple comparison of cur-SH and AaLS-IC-cur in PBS buffer with 0.8% ethanol, the solubility of AaLS-IC-cur is more than 30 times higher than cur-SH, that means loading of cur-SH into AaLS capsid can provide a better bioavailability.
4.3 The release of AaLS-IC-cur
About 60 % to 80 % of cur-SH can be free in a short time course when AaLS-IC-cur conjugate was treated with suitable reducing reagent (TCEP). While the hydrophobic property of cur-SH make more than 80 % free cur-SH cargos keep in hydrophobic cavity of capsid when cur-SH were saturated in aqueous solution. While the poor stability of cur-SH and AaLS-IC-cur in aqueous solution is a limitation for its further application.