Aim: To see the effect of caffeine on the heart rate of the water flea Daphnia.…
5. Establish the resting heart rate of the Daphnia. Count the amount of beats for ten seconds then multiply that number by six to convert it to beats/min.…
The following study was conducted in order to determine the effects of four different pharmaceutical compounds on the heart rate of Daphnia specimens. After basal heart rate was observed and computed, four different solutions each containing either an adrenergic, muscarinic, or nicotinic agonist or antagonist was tested against individual Daphnia. Observations were made through a compound light microscope through the 10x objective. It was found that nicotine decreased, caffeine increased, lidocaine decreased, epi decreased basal heart rate. This can be explained secondary to their effects as either receptor antagonists or agonists.…
An electrocardiogram, abbreviated as ECG in this report, is a visual representation of an organism’s atrial and ventricular depolarization and repolarization. Previous research has shown that although there is variability in the amplitude and shape of the waves of ECG, dependent on the subject’s health, it follows a universal pattern that can be used effectively for biometrics. The experiments discussed in this lab report were performed on Daphnia, humans and crayfish to demonstrate the effects of various stimulus on cardiac activity, i.e. heart rate. The data presented in this lab report was measured via ECG software for human and Crayfish cardiac activity, while the…
The purpose of lab number thirteen was to examine the response of the daphnia to a chemical stimulant. Also examined was how human resting heart rates differed from the daphnia’s. Stimulants are any substances that raise the levels of activity in the body. Stimulants could be a number of things such as physical or chemical. Physical stimulus usually deal with something that affects the five senses such as sight, hearing these could be attributed to loud noises or too much light going into your eye. Chemical stimuli differ because chemicals such as caffeine and alcohol usually cause these to affect cognitive abilities and heart rate. These same things can also affect muscle response and glandular response causing twitches and the production…
To improve this experiment the students could have added missing information, such as how many drops of each substance were added to the water, and the starting heart rate of the Daphnia. They also could have checked the heart rate a minute later to see if the substance has a lasting effect on the heart rate. An experiment that students could perform to verify the prediction that coffee…
* Calculate the heart rate, using the rapid rate calculation (counting the number of R waves in a 6-second strip and multiplying by 10 to calculate the heart rate per minute), for regular or irregular rhythms. For a regular rhythm, you can also use the precise rate calculation: Count the number of small squares between two consecutive R waves, and divide this number into 1,500 (the number of small squares in a 1-minute rhythm strip) to obtain the heart rate in beats per minute. Report the atrial and ventricular rates…
An experiment was held, on an aquatic invertebrate called ‘Daphnia’, to investigate the effect of caffeine on its heart rate. The fact that Daphnia is a transparent organism facilitates the experiment as it allows us to see its internal organs. Different caffeine solutions of different concentrations were used including one control solution without any caffeine in it at all. A caffeine solution is placed onto a Daphnia and the number of its heart beats within 10 seconds was counted. This procedure was repeated 10 times using the same concentration of caffeine but different Daphnia individuals each time. Afterwards this was repeated using different concentrations. All factors should be kept constant in order to be able to compare the results. The overall results were that as caffeine concentration increases, Daphnia’s heart rate increases too.…
In this laboratory experiment, our control was the Daphnia that gets spring water, whereas the drugs were the independent variable and the heart rate was the dependable variable. The first step our group did was to place the Daphnia into a clean depression slide. Next, we added a small drop of the spring water and put a cover slip over the slide. Then, we waited for one minute prior to placing the slide on the microscope in order to allow time for either solution or the drug to take its effect. After we put the slide on the microscope, we located the heart of Daphnia and counted its heartbeats while the other member kept time for each three 10-second trials. We recorded our data and calculated the average heart rates of three trials we found during the experiment. Afterwards, we calculated the average 60-second heart rates by multiplying the averages of our 10-second heart rates by 6. These procedures were repeated with the rest of solutions as well, including caffeine, aspirin, alcohol, sleep-aid, and…
Heart rate is also increased by adrenaline. Adrenaline is a hormone from the adrenal gland and it is released duringwhen we are doing exercise, which increases the heart rate, this is the sympathetic…
The autonomic nervous system controls the heart and has two branches; the sympathetic nervous system and the parasympathetic nervous system. When the body is undergoing muscular work, fear or stress the sympathetic nervous system will be active. When the sympathetic nervous system is active it will cause every heartbeat to increase in strength and heart rate. During resting, peace and contentment the parasympathetic nervous system is active and it calms the heart output. During periods of fright, flight and fight the sympathetic nervous system is boosted by the hormone; adrenaline. The nerves of the adrenaline are the cardiac nerves. A special cluster…
For this experiment we tested the effects of different adrenalin concentrations (0.001%; 0.01%; 0.1%) on the daphnia flea. The daphnia was placed on a slide with cover slip in order to examine its heartbeat by using the microscope.…
This occurs after feeding, during sleep, and during breath-holding and swimming underwater. A slowed heart rate and the associated fall in the rate of ejection of blood from the heart is sufficient to maintain body function during rest, and conserves energy in the heart under conditions where its supply (and the supply of oxygen in the blood) are diminished. A drug that slows heart rate is called a negative chronotrope and this is demonstrated in this experiment where acetylcholine is used to slow the rate of the Daphnia's…
To obtain the control of this experiment we prepared a slide with a single drop of water and a single Daphnia. We then counted the Daphnia 's heart rate for one minute.…
• Provide a few sentences of background information on the effects of these two drugs on heart rate in Daphnia or other animals (including humans). Cite the source(s) of your information, such as (Castle and Paulson 2010) for your laboratory manual. Also include the appropriate sources from your internet search. (See “Literature Cited” below.)…