1. Stage The horizontal surface upon which the slide is placed is called the stage. The slide is held in place by spring loaded clips and moved around the stage by turning the geared knobs on the stage. Th stage has two perpendicular scales that can be used to record the position of an object on a slide. This is useful if you want to quickly relocate an object.
2. Coarse adjustment The coarse adjustment knob located on the arm of the microscope moves the stage up and down to bring the specimen into focus. The gearing mechanism of the adjustment produces a large vertical movement of the stage with only a partial revolution of the knob. Because of this, the coarse adjustment should only be used with low power (4X and 10X objetives) and never with the high power lenses (40X and 100X). 3. Fine adjustment This knob is inside the coarse adjustment knob and is used to bring the specimen into sharp focus under low power and is used for all focusing when using high power lenses.
4. Ocular lens The ocular lens, or eyepiece, magnifies the image. It contains a measuring scale called and ocular micrometer. The ocular micrometer has no units.
5. Objective lens The objective lens gathers light from the specimen, magnifies the image of the specimen, and projects the magnified image into the body tube. Since no single objective lens can fulfill all the needs of someone using the microscope, several objective lenses of varying magnifications and numerical aperture are mounted on the rotation nosepiece. The nosepiece must click into place for the objective lens to be in proper alignmrnt. You will notice that the lower power lenses (4X and 100X) are shorter than the high power lenses (40X and 100X), meaning that the clearence between the objective lens and the stage is much smaller when the high power lenses are clicked into place. You must be very careful when using the high power lenses so