Deoxyribose Reaction Lab Report
Introduction
DNA, or deoxyribonucleic acid, is a double stranded helical structure used to store genetic information in cellular organisms. DNA usually consists of two strands made up of nucleotides, each with a backbone of repeating units of phosphate groups and the sugar, deoxyribose, bonded by phosphodiester linkages. Since the deoxyribose has a specific orientation, DNA molecules have directionality so that DNA sequences are read 5’ to 3’. The 5’ end of DNA is characterized by the deoxyribose ring that has a phosphate group attached to its fifth carbon, whereas the deoxyribose ring that has a terminating hydroxyl group attached to its third carbon characterizes the 3’ prime end1.
Connected to each deoxyribose in the backbone is one of …show more content…
After the addition of the ethanol, the DNA should precipitate as a spongy material that will wrap onto a glass rod. Lastly, the DNA on the glass rod was put in a test tube and placed in an 85° water bath5. The tissue used for the isolation of plant chromatin was raspberries. Two to three raspberries were mashed to form a pulp. The raspberry pulp was placed in a beaker to which an equal volume of a heated salt water/detergent solution was added. The mixture was stirred until it became homogeneous. The suspension was then poured over a beaker fitted with a coffee filter and secured with a rubber band, and spread using a depressor to properly filter the pulp from the filtrate. The filtrate was poured into a test tube until the test tube was 75% full. The test tube was then tilted to a 45° angle, and 95% ethanol was slowly poured in so that it dripped down the wall of the test tube, making sure not to shake the test tube. The ethanol formed a layer on top of the filtrate so that there were two layers in the test tube: one red (raspberry filtrate) and one colorless (ethanol). The DNA should have started to appear as a white viscous material. Lastly, a glass hook was used to wrap up the precipitated DNA by slowly swirling the glass hook …show more content…
DNA denaturation is a process that breaks apart the double stranded helical structure typical of DNA into two single DNA strands. This occurs by breaking the hydrogen bonds that hold the base pairs together in a double helix. A hindrance to DNA denaturation is base stacking; base stacking energies are so strong that they essentially cause adjacent bases to cohere. Since this step was not performed in the laboratory experiment due to the lack of viscous DNA, the DNA that precipitated into the ethanol remained intact in its double helix
DNA, or deoxyribonucleic acid, is a double stranded helical structure used to store genetic information in cellular organisms. DNA usually consists of two strands made up of nucleotides, each with a backbone of repeating units of phosphate groups and the sugar, deoxyribose, bonded by phosphodiester linkages. Since the deoxyribose has a specific orientation, DNA molecules have directionality so that DNA sequences are read 5’ to 3’. The 5’ end of DNA is characterized by the deoxyribose ring that has a phosphate group attached to its fifth carbon, whereas the deoxyribose ring that has a terminating hydroxyl group attached to its third carbon characterizes the 3’ prime end1.
Connected to each deoxyribose in the backbone is one of …show more content…
After the addition of the ethanol, the DNA should precipitate as a spongy material that will wrap onto a glass rod. Lastly, the DNA on the glass rod was put in a test tube and placed in an 85° water bath5. The tissue used for the isolation of plant chromatin was raspberries. Two to three raspberries were mashed to form a pulp. The raspberry pulp was placed in a beaker to which an equal volume of a heated salt water/detergent solution was added. The mixture was stirred until it became homogeneous. The suspension was then poured over a beaker fitted with a coffee filter and secured with a rubber band, and spread using a depressor to properly filter the pulp from the filtrate. The filtrate was poured into a test tube until the test tube was 75% full. The test tube was then tilted to a 45° angle, and 95% ethanol was slowly poured in so that it dripped down the wall of the test tube, making sure not to shake the test tube. The ethanol formed a layer on top of the filtrate so that there were two layers in the test tube: one red (raspberry filtrate) and one colorless (ethanol). The DNA should have started to appear as a white viscous material. Lastly, a glass hook was used to wrap up the precipitated DNA by slowly swirling the glass hook …show more content…
DNA denaturation is a process that breaks apart the double stranded helical structure typical of DNA into two single DNA strands. This occurs by breaking the hydrogen bonds that hold the base pairs together in a double helix. A hindrance to DNA denaturation is base stacking; base stacking energies are so strong that they essentially cause adjacent bases to cohere. Since this step was not performed in the laboratory experiment due to the lack of viscous DNA, the DNA that precipitated into the ethanol remained intact in its double helix