Preview

Determination of Quinine in Tonic Water

Good Essays
Open Document
Open Document
786 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Determination of Quinine in Tonic Water
Experiment 2

Title: Determination of Quinine in Tonic Water

Objective:
To Determine the Concentration of Quinine Hydrochloride in Sample (Schweppers) given, by plotting a Calibration Curve of Fluorescent Intensity against Concentration of Quinine Hydrochloride in ppm, after Fluorescence Intensity of a series of Standard Solution prepared and the Sample solution by Fluorescence Spectrometer.

Data:
Brand name of tonic water sample: Schweppes
Dilution factor = 1000

Concentration of quinine standard (ppm)
Fluorescence intensity (mean)
Blank
2.945
0.02
78.40
0.04
187.8
0.06
223.4
0.08
295.7
0.10
373.6
Original tonic water sample
2635
Diluted tonic water sample
273.3

Data Treatment:
1. Plot the calibration curve with fluorescent intensity against concentration of quinine hydrochloride in ppm (from Blank to 0.1 ppm).

By the Least Square Method, it is assumed that the relationship between x and y is linear, as below equation, y = mx + b m is the slope of equation and y is the y-intercept of equation

b = , m =

Solution
Concentration of Quinine Standard (ppm) (xi)
Fluorescence Intensity
(Mean) (yi)
(xi)2
xi yi
Blank
0.00
2.945
0.0000
0
0.02
0.02
78.4
0.0004
1.568
0.04
0.04
187.8
0.0016
7.512
0.06
0.06
223.4
0.0036
13.404
0.08
0.08
295.7
0.0064
23.656
0.10
0.10
373.6
0.0100
37.360
Sum of Column
0.30
1161.845
0.0220
83.500

m = = =3629.68 b = = = 12.157

By Least Square method, it was found that the relationship between Fluorescent Intensity against Concentration of Quinine Hydrochloride (in ppm) would be described as a linear equation of y = 3629.7x – 12.157 with R² = 0.9877

2. Report the brand name and the amount of quinine hydrochloride in ppm in the sample from the calibration curve.
The brand name of the sample: Schweppes

The corrected fluorescence intensity of sample = 14.542 y = 3629.7x – 12.157
273.3 = 3629.7x – 12.157 x =

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Chemistry 116 lab review

    • 289 Words
    • 2 Pages

    The first step is to calibrate the colorimeter with0.20 M Fe(NO3)3and set the absorbance at 470 nm since it is known to keep an acidic solution throughout the entirety of the experiment. It was important to do this right at the beginning of the lab since the zeroed value of the acid was the calibration number for all of the other solutions. A total of seven solutions with different dilutions were used throughout the lab to conduct the equilibrium constant. The first step was adding 5 mL of 0.200 M Fe(NO3)3to each of the 5 test tubes. Once this was done, 0.00200 M NCS was added to the test tubes, each receiving a different amount; test tube one received 1 mL NCS-and with each test tube the amount of NCS-would increase by 1 mL, test tube 5 received 5 mL of NCS. . The next step was adding HNO3 to each test tube in different volumes; Test tube one received 10 mL of HNO3 and with each test tube the amount of HNO3 decreased by 1 mL, test tube five had no HNO3 added to it. The addition of these solutions formed five test tubes of different dilutions, but of equal volume, 10 mL each. After all of the previous trials had been completed the final step was to take each test tube and pour it into a different cuvette and measure the absorbance for each. Once the initial concentration was calculated of Fe3+, NCS and FeNCS2+ in molarity. The absorbency values were recorded and used to calculate the formation constant, K f The reference table containing volumes used in each solution is provided below…

    • 289 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    CHEM 1252 Lab Report 5

    • 1825 Words
    • 21 Pages

    The absorbance of a small test tube filled with 4.0 mL of the 0.2 M NaOH solution and 1 drop of phenolphthalein was recorded every 5 seconds for 360 seconds. Then, ln(absorbance) and 1/(absorbance) were calculated for these values…

    • 1825 Words
    • 21 Pages
    Good Essays
  • Good Essays

    Experiment 3 prelab

    • 345 Words
    • 2 Pages

    The objectives of this lab include- illustrating the use of the spectrophotometer in chemical analysis, and generating a standard, or calibration curve, then using that curve to determine the value of an unknown substance. The spectrophotometer is one of the most powerful tools used in chemistry to find the concentration of substances in solution. It compares the colors of a known and an unknown solution, that comparison then leads to a quantitative estimate of the concentration of trace amounts of colored materials in that solution.…

    • 345 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Chem Lab.

    • 520 Words
    • 3 Pages

    13. Observe your vial and the contents. Make a drawing in the space provided of the vial and its contents and label all chemicals.…

    • 520 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Food Dyes Lab

    • 671 Words
    • 3 Pages

    The initial step to determine the concentration of the dyes previously found was to prepare a set of serial dilutions at 20%/40%/60%/80%/100% dye concentration. To prepare the 20% dilution 1mL of red 40/blue 1 was combined with 4mL of DI water. This method was used to produce the remaining dilutions with 2mL/3mL, 3mL/2mL, 4mL/1mL, and 5mL/0mL (mL dye / mL DI water). Each of the previous dilutions was tested in the spectrometer and data was recorded. Using the data obtained two Beer Law plots were plotted for both dyes and trendlines were added. The slope of the trendline was found and used to calculate the actual (mol/L) and percent concentrations…

    • 671 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    The purpose of this experiment was to observe the color change and the concentration to determine the…

    • 1426 Words
    • 6 Pages
    Satisfactory Essays
  • Good Essays

    To measure the color intensity, you will be using a Colorimeter or Spectrometer. In this device,…

    • 476 Words
    • 2 Pages
    Good Essays
  • Better Essays

    We found the Rf value by the distance spot travels divided by the distance the solvent travels. From the TLC samples collected, the solvent travels to be 7.7 cm and the distance the spot travels from aspirin, acetaminophen, ascorbic acid, and unknown to be 7.0 cm, 7.7 cm, 7.6 cm, and 7.5 cm respectively. Since the two…

    • 990 Words
    • 4 Pages
    Better Essays
  • Better Essays

    | * Wooden Peg * 10 ml Measuring cylinder * Curvette * Spectrophotometer * Gold Solution (HAuCl4) * Heat proof mat…

    • 1155 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Peroxidase Lab

    • 823 Words
    • 4 Pages

    The materials needed for this experiment were four medium sized tubes, a spectrophotometer, a buffer with a pH of 5, H2O2, Peroxidase, and Guaiacol Dye. We as a group had four tubes labeled two, three, four, and five. In the tube labeled two we had a solution of 2.0 mL of H2O2 and 1.0 mL of Guaiacol Dye for a total solution of 3.0 mL. In the tube labeled three we had a solution of 4.0 mL buffer and 1.0 mL Peroxidase for a total of 5.0 mL solution. In the tube labeled four we had a solution of 2.0 mL H2O2 and 1.0 mL Guaiacol Dye for a total of 3.0 mL solution. Last, in the tube labeled five we had a solution of 4.0 mL buffer and 1.0 mL Peroxidase for a total solution of 5.0 mL. We then set the spectrophotometer to a value of zero, using a blank containing buffer, H2O2, and Guaiacol Dye. After that, tubes two and three were mixed together and tubes four and five were mixed together for a large solution of with a total of 8.0 mL. Once the two solutions are mixed clean the tube with a Kim Wipe, so all fingerprints are removed. Place the tube with solutions of four and five in the ice bath for twenty minutes. While placing the tube in the spectrophotometer make sure the white strip that is on the tube is facing the direction of the person who is handling placing the tube I the spectrophotometer. Furthermore, just let the spectrophotometer act on the solution for the desired length of time while recording the data at each specific…

    • 823 Words
    • 4 Pages
    Good Essays
  • Good Essays

    In order to complete the objective, the group used a Carolina Digital Spectrophotometer, unit M 201-1, which measures the quantity of light that solutions absorb. The spectrophotometer consists of a light source that shines through a prism which then shines through a…

    • 1159 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Then approximately 0.8 g of KHP was placed in the flask. 100 mL of water was added and was swirled until dissolved. Three drops of phenolphthalein indicator were then added.…

    • 716 Words
    • 3 Pages
    Good Essays
  • Better Essays

    protocal

    • 987 Words
    • 4 Pages

    2. Learn the procedure for constructing a standard curve and practice using the standard curve to determine concentration of unknown samples.…

    • 987 Words
    • 4 Pages
    Better Essays
  • Satisfactory Essays

    The 2nd graph was between concentration and absorbance. This is a direct relationship because as the concentration increased, the absorbance also increased. For this graph, the line should touch the origin because it is a positive slope going from lower values to higher values. Also it passes through the origin because direct variation relationships are in the form of y = mx, where y and m are constant variables. For the absorbance value to zero, the concentration must be also be zero.…

    • 304 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Experiment 2

    • 532 Words
    • 3 Pages

    Figure 1. Sample calibration curve of standard Fluorescence solutions, average absorbance against concentration of standards. The concentration range of standard used was from 4.984x10-9 to 49.84ppm. The best fit line was determined by Regression analysis in Microsoft® Excel. The equation of best fit was y=0.1255 x +0.3353, the slope was 0.1255 V/ppm and the y intercept was 0.3353 V. The R2 value was 0.965.…

    • 532 Words
    • 3 Pages
    Satisfactory Essays