Preview

Determination Of Total Carbohydrateby Anthrone Method

Satisfactory Essays
Open Document
Open Document
369 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Determination Of Total Carbohydrateby Anthrone Method
Determination of Total Carbohydrateby Anthrone Method

Carbohydrates are the important components of storage and structural materials in the plants.They exist as free sugars and polysaccharides. The basic units of carbohydrates are themonosaccharides which cannot be split by hydrolysis into more simpler sugars. The carbohy-drate content can be measured by hydrolysing the polysaccharides into simple sugars by acidhydrolysis and estimating the resultant monosaccharides.

PRINCIPLE

Carbohydrates are first hydrolysed into simple sugars using dilute hydrochloric acid. In hot acidic medium glucose is dehydrated to hydroxymethyl furfural. This compound forms with anthrone a green coloured product with an absorption maximum at 630 nm.

MATERIALS
2.5 N HCl
Anthrone reagent: Dissolve 200 mg anthrone in 100 ml of ice-cold 95% H2SO4. Prepare fresh before use.
Standard glucose: Stock —Dissolve 100 mg in 100 ml water. Working standard— 10 ml of stock diluted to 100 ml with distilled water. Store refrigerated after adding a few drops of toluene.

PROCEDURE
1- Weigh 100 mg of the sample into a boiling tube.
2- Hydrolyse by keeping it in a boiling water bath for three hours with 5 mL of 2.5 N HCl and cool to room temperature.
3- Neutralise it with solid sodium carbonate until the effervescence ceases.
4- Make up the volume to 100 mL and centrifuge.
5-Collect the supernatant and take 0.5 and 1 mL aliquots for analysis.
6- Prepare the standards by taking 0, 0.2, 0.4, 0.6, 0.8 and 1 mL of the working standard.‘0’serves as blank.
7-Make up the volume to 1 mL in all the tubes including the sample tubes by addingdistilled water.
8-Then add 4 mL of anthrone reagent.
9- Heat for eight minutes in a boiling water bath.
10- Cool rapidly and read the green to dark green colour at 630 nm.
11- Draw a standard graph by plotting concentration of the standard on the X-axis versus absorbance on the Y-axis.
12- From the graph calculate the amount of carbohydrate present in the sample tube.

You May Also Find These Documents Helpful

  • Good Essays

    Carbohydrate – sugars, encompasses the monomers, called monosaccharides, small polymers called oligosaccharides, and large polymers called polysaccharides…

    • 712 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Chem Lab.

    • 520 Words
    • 3 Pages

    3. Determine the mass of the clean dry vial and record your mass to 2 decimal places on your lab sheet.…

    • 520 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    3. Add 10-20 drops of each known solution to respective test tubes, do not mix pipets!…

    • 372 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Dye Analysis Lab Report

    • 978 Words
    • 4 Pages

    5) Using a serological pipet for accurate volume measurements, dilute the stock solution as indicated in the following table to prepare 10 mL each of a series of standard solutions, B-H. Thoroughly mix each solution. Hint: To avoid contaminating the stock solution, and then measure and add the required amount of stock solution to each test tube.…

    • 978 Words
    • 4 Pages
    Satisfactory Essays
  • Satisfactory Essays

    1. Prepare a 50% NaOH solution by dissolving 1.0 g NaOH in 1 mL of water.…

    • 696 Words
    • 4 Pages
    Satisfactory Essays
  • Powerful Essays

    7. Refill half of the test tube with room temperature distilled water (at least 10C) and add 1/8 teaspoon of salt to the distilled water in the test tube. Mix well…

    • 1713 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    Ap Bio Study Guide

    • 4984 Words
    • 20 Pages

    Carbohydrates 1) Monosaccharides= same formula (C6H12O6) yet slightly different structures 2) Polysaccharides a. Starch (amylose)= unbranched chain of glucose b. Glycogen= branched chain of glucose (20 min supply in muscles) both starch & glycogen are made from α (alpha) glucose, both bonds can be hydrolyzed by our bodies c. Cellulose is made from β (beta) glucose. Our bodies cannot hydrolyze cellulose: cellulose is indigestible & comes out as #2. β Glucose are H‐bonded together for added strength α Glucose does not have H‐bonds Lipids 1) Triglyceride a. Glycerol + 3 fatty acid molecule 3 carbons | each fatty acid is a long chain b. Saturated= all carbon‐carbon bonds are single (c‐c), solid at room temperature c. Unsaturated= at least one carbon‐carbon bond is double (c=), Liquid…

    • 4984 Words
    • 20 Pages
    Good Essays
  • Good Essays

    water on the beaker with sodium carbonate. Use a stirring rod to stir it and then we you…

    • 612 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Bla to the Bla Bla Bla

    • 434 Words
    • 2 Pages

    Bring 5 quarts of water to boil and dissolve the sugar in the water. Remove from heat and add frozen concentrate. Add additional water to make five gallons and pour into secondary. Add remaining ingredients except yeast. Cover with cloth fastened with rubber band and set aside 12 hours. after cooling to proper yeast temperature, add activated yeast and recover with…

    • 434 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    9. Fill your cylinder with enough water so that when the sample is dropped in, it will be completely submerged (80mL was used in this experiment).…

    • 836 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Simple Sugar Lab

    • 608 Words
    • 7 Pages

    4. Label all test tubes. obtain a graduated cylinder. Add 10 mL of a different food…

    • 608 Words
    • 7 Pages
    Good Essays
  • Good Essays

    Chemical Reactions Lab

    • 651 Words
    • 3 Pages

    beaker with up to 100 mL mark with distilled water. Heat the solution and allow…

    • 651 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Procedure 1. Using syringes make up the following mixtures in five dry 100 cm3 beakers.…

    • 1498 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    9. Use a new syringe to extract a 10 ml sample from test tube B and place it in dish B and repeat for test tube C/dish C.…

    • 654 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Cyclohexanone Lab Report

    • 420 Words
    • 2 Pages

    Be sure to stir the mixture for 2 minutes after adding sodium thiosulfate. Repeat the potassium iodide-starch test to make sure that there are no oxidize agent left in the mixture. Once done, the mixture will be tested for it pH level. If the mixture is too acidic, then add sodium bicarbonate, as necessary, to reduce acidity. In the next step, add enough sodium chloride so that a small amount will remain after stirring. This will decrease the solubility of organic compounds and increase the amount of product that can be collected. Decant the liquid part of the mixture into a separatory funnel. Wait 5 minutes for the layers to separate and then drain the bottom layer into the Erlenmeyer flask and the top layer into a vial. Use a small pipette to take out any bubble in the vial. Add a small amount of sodium sulfate, a drying agent, to the vial and let it sit for 5…

    • 420 Words
    • 2 Pages
    Satisfactory Essays