Dna Fingerprinting Lab Report
Introduction
For this experiment we compared and analyzed various DNA fragments in order to determine if these DNA fragments originated from the same individual. The learning objective for this lab is to gain a better understanding of how DNA fingerprinting works. In this lab the primary function is to determine which DNA fragments match the DNA fragment found on the crime scene. To determine if any of the DNA fragments match the fragment found at the crime scene, the DNA fragments must undergo the DNA fingerprinting. DNA fingerprinting causes the sugar-phosphate backbone bond at specific sites in the nucleotide bases to break. The destruction of these bonds is caused by restriction enzymes. These restriction enzymes have designated recognition …show more content…
After this binding it splits the DNA into two strands, and then restriction endonucleases, “cut” out parts of the DNA strand. As a result of this cutting one is left with their DNA fingerprint which is unique to each individual. My prediction for this experiment is that at least one of the DNA fragments will match up the DNA fragment that was found at the scene of the crime.
Materials and Methods
The first step of the DNA fingerprinting process is to collect the 10µL of DNA samples from the Crime Scene (CS), Suspect 1(S1), Suspect (2), and Suspect 3(S3). Once you have your DNA samples, using a pipette add 10µL of the enzyme mix into each DNA tube. When doing this it is important to note that you must change the pipette tip after every use to ensure there is no cross contamination. As soon as both the DNA sample and the enzyme are in the tube, pipette up and down to mix the solution. Following this step, close the caps on the tubes and mix the contents of the tube by carefully flicking the tubes with a finger. Then place the tubes in a balanced manner in the microcentrifuge and spin for approximately five seconds. This will force the liquid to the bottom of the tubes. After removing the tubes from the microcentrifuge place them in a foam micro tube holder
For this experiment we compared and analyzed various DNA fragments in order to determine if these DNA fragments originated from the same individual. The learning objective for this lab is to gain a better understanding of how DNA fingerprinting works. In this lab the primary function is to determine which DNA fragments match the DNA fragment found on the crime scene. To determine if any of the DNA fragments match the fragment found at the crime scene, the DNA fragments must undergo the DNA fingerprinting. DNA fingerprinting causes the sugar-phosphate backbone bond at specific sites in the nucleotide bases to break. The destruction of these bonds is caused by restriction enzymes. These restriction enzymes have designated recognition …show more content…
After this binding it splits the DNA into two strands, and then restriction endonucleases, “cut” out parts of the DNA strand. As a result of this cutting one is left with their DNA fingerprint which is unique to each individual. My prediction for this experiment is that at least one of the DNA fragments will match up the DNA fragment that was found at the scene of the crime.
Materials and Methods
The first step of the DNA fingerprinting process is to collect the 10µL of DNA samples from the Crime Scene (CS), Suspect 1(S1), Suspect (2), and Suspect 3(S3). Once you have your DNA samples, using a pipette add 10µL of the enzyme mix into each DNA tube. When doing this it is important to note that you must change the pipette tip after every use to ensure there is no cross contamination. As soon as both the DNA sample and the enzyme are in the tube, pipette up and down to mix the solution. Following this step, close the caps on the tubes and mix the contents of the tube by carefully flicking the tubes with a finger. Then place the tubes in a balanced manner in the microcentrifuge and spin for approximately five seconds. This will force the liquid to the bottom of the tubes. After removing the tubes from the microcentrifuge place them in a foam micro tube holder