Dynamic Light Scatteringn Case Study
3.1 Overview
The experiment was divided into three parts. In the first part was the preparation of empty CNPs. The second part was the preparation of CNPs loaded with single drug which was AA and TQ meanwhile the third part was the preparation of CNPs loaded with dual drug (AA and TQ). The last part was the analytical testing conducted to investigate the properties performance such as smaller or larger in trend as well as to characterize the entrapment efficiency of drugs. The chemicals and technique used to characterize the CNPs nanoparticles are listed and the instruments used to characterize the CNPs nanoparticles are discussed.
3.2 Materials
Chitosan with MW= 50,000-190 000 Da will be used as a carrier (Sigma-Aldrich, UK). …show more content…
A low polydispersity index (PDI) value corresponding to more monodisperse CNPs. The measurement was repeated three times for each sample and the average of the three measurements was calculated. All values of the mean particle size distribution and PDI value were expressed as the mean ± standard deviation (S.D.).
3.3.3 Fourier transform infrared (FTIR)
FTIR was performed using a Perkin Elmer 1725X Spectrometer. The significant transmittance peak at particular wavenumbers was measured by using the “ find peak tool” provided by Nicolet OMNIC 5.01 software. The empty CNP, CNP loaded with AA, CNP loaded with TQ and CNP loaded with AA and TQ were dispersed and mixed with the KBr particles together and compressed into pellets. The spectrum of chitosan nanoparticles recorded in the range of 4000-280 cm-1.
3.3.4 Entrapment Efficiency …show more content…
The FESEM uses a beam of electrons shot towards a sample to obtain information about it such what the surface looks like and the chemical makes up of the sample. Nanoparticles were diluted prior to analysis by mixing 100 μL of samples with 500 μL of distilled water. A drop of each diluted samples was then placed onto an aluminium stub and left to dry in an oven for 72 hours. The sample-loaded stubs were then gold-coated in a vacuum before being observed under an electron microscope (FEI NOVA nanoSEM 230, Thermo Fisher, Hillsboro, OR,
The experiment was divided into three parts. In the first part was the preparation of empty CNPs. The second part was the preparation of CNPs loaded with single drug which was AA and TQ meanwhile the third part was the preparation of CNPs loaded with dual drug (AA and TQ). The last part was the analytical testing conducted to investigate the properties performance such as smaller or larger in trend as well as to characterize the entrapment efficiency of drugs. The chemicals and technique used to characterize the CNPs nanoparticles are listed and the instruments used to characterize the CNPs nanoparticles are discussed.
3.2 Materials
Chitosan with MW= 50,000-190 000 Da will be used as a carrier (Sigma-Aldrich, UK). …show more content…
A low polydispersity index (PDI) value corresponding to more monodisperse CNPs. The measurement was repeated three times for each sample and the average of the three measurements was calculated. All values of the mean particle size distribution and PDI value were expressed as the mean ± standard deviation (S.D.).
3.3.3 Fourier transform infrared (FTIR)
FTIR was performed using a Perkin Elmer 1725X Spectrometer. The significant transmittance peak at particular wavenumbers was measured by using the “ find peak tool” provided by Nicolet OMNIC 5.01 software. The empty CNP, CNP loaded with AA, CNP loaded with TQ and CNP loaded with AA and TQ were dispersed and mixed with the KBr particles together and compressed into pellets. The spectrum of chitosan nanoparticles recorded in the range of 4000-280 cm-1.
3.3.4 Entrapment Efficiency …show more content…
The FESEM uses a beam of electrons shot towards a sample to obtain information about it such what the surface looks like and the chemical makes up of the sample. Nanoparticles were diluted prior to analysis by mixing 100 μL of samples with 500 μL of distilled water. A drop of each diluted samples was then placed onto an aluminium stub and left to dry in an oven for 72 hours. The sample-loaded stubs were then gold-coated in a vacuum before being observed under an electron microscope (FEI NOVA nanoSEM 230, Thermo Fisher, Hillsboro, OR,