Effect of Detergent Concentration on Membrane Permeability of Beetroot Cells
Aim: To investigate how effect of Detergent Concentration (cont.) has on Membrane permeability of Beetroot cells.
Hypothesis: I predict that as detergent concentration increases, the solution will become less clear, plus mass increases. The increases in mass will indicate that the water potential of the Beetroot cell is lower than that of the surrounding sucrose solution. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than the water potential inside the beetroot cell. I predict that Osmosis will not occur (at 0% cont.) if the water potential of the sucrose solution (detergent) and the water potential of the cell’s cytoplasm are equal. I also predict that an increase in detergent concentration will lead to more damage to the cell membranes, decreasing their permeability, and therefore allowing more of the pigment to be released. I predict that if the concentration of detergent is doubled, double the cells will be (at least partly) damaged, which will lead to double the amount of beta cyanine being let out of the cells.
Control Variables: In order to make the test fair, only one person must time the amount of time the beetroot has been inside the concentrated detergent and the distilled water, to make the test more accurate because different people have different reaction times, so by using the same person to time the amount of time the beetroot has been inside the concentrated detergent and the distilled water, the reaction time is not change. The same person should always cut the beetroot because different people have different eye quality, so by using the same person to cut the beetroot will result to a fair test, also when cutting to be sure to use the same ruler and making sure the cutting devices that is being use is washed before cutting the next 1 out of 4,1cm length beetroot. The beetroot should always be washed to remove the pigment leaked from damaged cells. Each test tube should
Hypothesis: I predict that as detergent concentration increases, the solution will become less clear, plus mass increases. The increases in mass will indicate that the water potential of the Beetroot cell is lower than that of the surrounding sucrose solution. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than the water potential inside the beetroot cell. I predict that Osmosis will not occur (at 0% cont.) if the water potential of the sucrose solution (detergent) and the water potential of the cell’s cytoplasm are equal. I also predict that an increase in detergent concentration will lead to more damage to the cell membranes, decreasing their permeability, and therefore allowing more of the pigment to be released. I predict that if the concentration of detergent is doubled, double the cells will be (at least partly) damaged, which will lead to double the amount of beta cyanine being let out of the cells.
Control Variables: In order to make the test fair, only one person must time the amount of time the beetroot has been inside the concentrated detergent and the distilled water, to make the test more accurate because different people have different reaction times, so by using the same person to time the amount of time the beetroot has been inside the concentrated detergent and the distilled water, the reaction time is not change. The same person should always cut the beetroot because different people have different eye quality, so by using the same person to cut the beetroot will result to a fair test, also when cutting to be sure to use the same ruler and making sure the cutting devices that is being use is washed before cutting the next 1 out of 4,1cm length beetroot. The beetroot should always be washed to remove the pigment leaked from damaged cells. Each test tube should