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Effect Of Sucrose Concentration On Osmolality Of Plant Cells

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Effect Of Sucrose Concentration On Osmolality Of Plant Cells
Christine Otieno
Estimating Osmolarity of plant Cells(potato and celery)by percentage change in weight Using different sucrose concentrations
Introduction
The purpose of this experiment was to estimate Osmolality of plants cells i.e. Potato and celery by converting the observed change of weight in different sucrose concentrations. The hypothesis assumes that the solute concentration of the plant samples would be indirectly proportional to the weight; there would be decrease in weight as the concentration increases. The independent variable in this experiment would be the change in weight while the independent variable would be the concentration of sucrose. The control group used is the weight of both potato and celery before emersion into the sucrose solution. The constant control variables were the potato and celery.
Materials (Entire lab class) * Large potato tuber,3 celery stalks * 24 test tubes(4 tubes per group of four students) * 6 petri dish(1 dish per group) * Wax marking pencils,paper towels * Forceps,balance,Aluminium foil,razer blade,knives * 100mls sucrose solutions: 0.1, …show more content…
This was calculated using the percentage weight formula and concentration in moles per mls while the weight was measured in grams. Time was also noted in hours and minutes. In dilute concentrations the celery looked turgid due to the hypotonic conditions they had more water in them. But as concentrations changed to hypertonic the samples shrunk because they had less water. Isotonic conditions were observed at a molarity of0.32M in the celery and at 0.36M in the potato. At 0M the potato and celery had percentage weight change of 17.07% and 9.93% respectively. The percentage in weight kept falling and rising inconsistently, At 0.33M and 0.5M both samples had almost the same percentage weight
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