Effect of temperature and SDS concentration on cell membranes of beet root cell
EFFECT OF CHANGES IN TEMPERATURE AND SDS CONCENTRATION ON THE STRUCTURE OF BEETROOT CELL MEMBRANES.
Introduction
Cellular Membranes or cell membranes are biological membranes that separate the interior of a cell from the outside environment. Cell membranes are selectively permeable to ions and some molecules and control the movement of substances in and out of the cell. (Danyk, 2013/14)
Cell membranes separate and organize chemicals and reactions within cells by allowing selective passage of materials across their boundaries. They are composed of a bilayer of phospholipid molecules interspersed with protein molecules. Most membranes also contain very small amounts of carbohydrates that are usually associated with the phospholipids or proteins. (Danyk, 2013/14)
Phospholipids in the membrane are composed of a phosphate group, glycerol backbone and 2 fatty acid chains. They are amphipathic; each has a hydrophobic region and a hydrophobic region. The nonpolar lipid tails are hydrophobic and the polar phosphate and glycerol group are hydrophilic. (Danyk, 2013/14)
Protein molecules are interspersed within the membrane. Relatively weak hydrogen bonds hold the membrane proteins in specific folded conformations. The physical and chemical study integrity of a membrane is crucial for the proper functioning of the cell or the organelle of which it is a part. A membrane’s permeability is related to its phospholipids and transport proteins. (Danyk, 2013/14)
This study involves observing the effects of temperature and detergent concentration (%SDS) on beet cell membrane integrity and how the loss of membrane integrity leads to loss of membrane function. (Danyk, 2013/14) SDS increases the staining intensity(Brown, Lydon et al. 1996) since it removes the proteins embedded in the cell membrane(Freeman, 2002) and temperature would affect the arrangements of the phospholipids by increasing fluidity or retaining movement.
A break in the integrity of the
Introduction
Cellular Membranes or cell membranes are biological membranes that separate the interior of a cell from the outside environment. Cell membranes are selectively permeable to ions and some molecules and control the movement of substances in and out of the cell. (Danyk, 2013/14)
Cell membranes separate and organize chemicals and reactions within cells by allowing selective passage of materials across their boundaries. They are composed of a bilayer of phospholipid molecules interspersed with protein molecules. Most membranes also contain very small amounts of carbohydrates that are usually associated with the phospholipids or proteins. (Danyk, 2013/14)
Phospholipids in the membrane are composed of a phosphate group, glycerol backbone and 2 fatty acid chains. They are amphipathic; each has a hydrophobic region and a hydrophobic region. The nonpolar lipid tails are hydrophobic and the polar phosphate and glycerol group are hydrophilic. (Danyk, 2013/14)
Protein molecules are interspersed within the membrane. Relatively weak hydrogen bonds hold the membrane proteins in specific folded conformations. The physical and chemical study integrity of a membrane is crucial for the proper functioning of the cell or the organelle of which it is a part. A membrane’s permeability is related to its phospholipids and transport proteins. (Danyk, 2013/14)
This study involves observing the effects of temperature and detergent concentration (%SDS) on beet cell membrane integrity and how the loss of membrane integrity leads to loss of membrane function. (Danyk, 2013/14) SDS increases the staining intensity(Brown, Lydon et al. 1996) since it removes the proteins embedded in the cell membrane(Freeman, 2002) and temperature would affect the arrangements of the phospholipids by increasing fluidity or retaining movement.
A break in the integrity of the
Cited: Campbell, N.A., Reece, J.B. (2011). Campbell Biology, ninth edition. Pearson Education Inc., San Francisco, CA. Danyk, Helena. The cellular basis of life laboratory manual. University of Lethbridge, Lethbridge, Alberta. Freeman, Scott, 2002, Biological Sciences, Prentice Hall Upper, Upper Saddle River, NJ Brown, D., et al. (1996). "Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS)." Histochemistry and Cell Biology 105(4): 261-267. McNeil, P. L. and R. A. Steinhardt (1997). "Loss, Restoration, and Maintenance of Plasma Membrane Integrity." The Journal of Cell Biology 137(1): 1-4.