Enterotube 2 Lab Report

Microbiology Sec 147848
November 18, 2013 Title of the Experiment: Enterobacteriaceae Identification: The Enterotube II System
Learning Objectives: After completing this exercise we were able to inoculate an unknown bacterium that belongs to the Enterobacteriaceae by using technology effectively with a Enterotube II. An Enterotube II is a miniaturized multi-test system for rapid identification of enterbacteriaceae. We then evaluated the test results and generated a five-digit code for the unknown bacterium. Thinking creatively and critically we had to figure out the code by looking at the Enterotube and comparing the reactants to the original to see what the result was. We then had to use the five-digit code to correctly identify the
Any sign of yellow should be interpreted as a positive reaction, orange should be considered negative. Lactose Bacterial fermentation of lactose, which results in the formation of acidic end products, is indicated by a change in color of the Indicator present in the medium from red (alkaline) to yellow (acidic). Any sign of yellow should be interpreted as a positive reaction; orange should be considered negative. Arabinose Bacterial fermentation of arabinose, which results in the formation of acidic end products, is indicated by a change in color from red (alkaline) to yellow (acidic). Any sign of yellow should be interpreted as a positive reaction; orange should be considered negative. Sorbitol Bacterial fermentation of sorbitol, which results in the formation of acidic end products, is indicated by a change in color from red (alkaline) to yellow (acidic). Any sign of yellow should be interpreted as a positive reaction; orange should be considered negative. Voges-Proskauer Acetylmethylcarbinol (acetoin) is an intermediate in the production of butylene glycol from glucose fermentation. The presence of acetoin is indicated by the development of a red color within 20 minutes. Most positive reactions are evident within 10 minutes. Phenylalanine Deaminase This test detects the formation of pyruvic acid from the deamination of phenylalanine. The pyruvic acid formed reacts with a ferric salt in the medium to produce a characteristic black to smoky gray color. Urea The production of urease by some bacteria hydrolyzes urea in this medium to produce ammonia, which causes a shift in pH from yellow (acidic) to reddish-purple (alkaline). This test is strongly positive for Proteus in 6 hours and weakly positive for Klebsiella and some Enterobaeter species in 24