Enzyme Lab Report Essay

After conducting the experiment and gathered the required data, we came to conclusion that certain environmental factors affect the enzyme activity rate. For the first experiment, where we tested the increase in concentration of enzyme with the substrate, we found that higher concentration of enzyme increases the rate of reaction of the enzyme. This is because more enzyme molecules are present, which allow more substrate molecules to get into the active sites of the enzyme (Sattler W& Esterbauer H). When calculating the absorbance of different enzyme concentration, it was noticeable that the absorbance decreased as time increased; however, at a certain time it decreased and then increased. This might have occurred because we have done something wrong either we added more solution or the cuvette had finger print. However, our results confirm our hypothesis that higher concentration of enzyme cause an increase in the rate of reaction at a certain point. The graph of “Enzyme's Concentration Effect on a Substrate Concentration “illustrates the slopes of the lines of different enzyme concentration. It is apparent that the slope of the % amylose of undiluted enzyme was fluctuating, whereas, the % amylose of 1:9 undiluted enzyme
was high and remained high. Furthermore, from the graph and data we collected of the effect of pH levels on the rate of reaction we drew a conclusion that the optimum level of pH, at which the solution will have the highest rate of reaction among the other pH levels, is pH 6.
The reason behind that is because changes in pH level change the shape of the enzyme which ultimately affect the effectiveness of the rate of reaction, either increase it or decrease it. Lower or higher pH level than pH 6 will lower the rate of reaction. Looking at the graph of “Enzyme Effected by pH Levels” we can see the slope of pH6 was increasing and then it gradually decreased. One of the limitation we encountered was the inaccuracy of the data collected, which we solved by redoing the
experiment.
The effect of temperature on the rate of enzyme reaction was determined. From our observation and data gathering we can prove our hypothesis of the effect of higher temperature on the rate of reaction. As the temperature rises the enzyme molecules have higher kinetic energy, which increases the speed of combing the substrates to the active sites. Then the enzyme will reach a point, at which the reaction will reach its maximum, after that, an increase in temperature will alter the structure of the enzyme, which causes it to denture (Sattler W& Esterbauer H). From our experiment we found out that the reaction will have highest enzyme activity at the optimum temperature of amylase, which is 22°C. Any temperature above the optimum temperature will change the shape of the enzyme causing the active site to change as well, which does not allow more substrate to enter the active site.
Our data can be quite beneficial in further studies involving enzyme