Eukaryote Model Research Papers

The social amoeba Dictyostelium discoideum is an interesting microbe that has used as a powerful model organism for biomedical and pharmacological studies. This eukaryote model, which has been used to study basic cell function and multicellular development, undergoes a 24-h asexual life cycle comprised of both single-cell and multicellular phases. As a result, it is an excellent system for studying a variety of cellular and developmental processes such as, chemotaxsis, lysosome function and signaling. In nature, Dictyostelium feeds and grows as single cells. When induced by starvation, cells undergo chemotactic aggregation towards cAMP to form a multicellular aggregate (i.e., a mound), which then undergoes a series of morphological changes to form a motile multicellular pseudoplasmodium, also referred to as a slug. Cells within the slug then terminally differentiate into either stalk or spore to form a fruiting body.
Ibuprofen (2-(4-isobutylphenyl) propionic acid, is a Nonsteroidal anti-inflammatory drugs (NSAID), which is commonly used for the treatment of pain and inflammation. The concentration of ibuprofens found on drug stores is 100 mg of ibuprofen in 5 mL (20 mg/mL), and the molecular weight is 206.28 (drugs.com). It is one of the most potent orally and highly active
discoideum transition from growth to development stage. As a result, GST activity will be determined using 1-chloro-2,4-dinitrobenzene (CDNB) substrate. Sonication of the cells in 100 mM potassium phosphate buffer (pH 6.2) with protease inhibitor cocktail (PIC). The GST reaction contained final concentrations of 97 mM potassium phosphate, 2.5 mM reduced glutathione, 1.0 mM CDNB, and 20 mg protein. Absorbance at 340 nm will be recorded at 60 s intervals for 10 min. GST enzyme activity will be validated by inhibition with 500 nM ethacrynic acid under reaction conditions as described above. Activity was recor- ded as