Experiment 1 Protocol
General Lab Safety
Experiment 1: Neutralization of Acids and Bases
In this experiment, you will learn how to properly neutralize and dispose of acidic and basic solutions.
Materials
5 mL 4.5% Acetic Acid (vinegar), C2H4O2
2 Pipettes
(1) 250 mL Beaker
0.5 g Sodium Bicarbonate (baking soda), NaHCO3
(1) 10 mL Graduated Cylinder
2 Weigh Boats
(1) 100 mL Graduated Cylinder
*Water
8 Litmus Test Strips (Neutral)
Permanent Marker
*You Must Provide
Procedure
1. Use the permanent marker to label two of the weigh boats as A and C.
2. Use the permanent marker to label a 250 mL beaker B.
3. Set the containers in the order A, B, and C.
4. Measure and pour approximately 5 mL of water into weigh boat “A”.
5. Add 0.5 g sodium bicarbonate to beaker “B”.
6. Use the 100 mL graduated cylinder to measure and pour 100 mL of water into beaker “B”. Gently pipette the solution up and down until the sodium bicarbonate is fully dissolved in the water. Leave this pipette in beaker B to mix the solution in future steps.
7. Use the 10 mL graduate cylinder to measure and pour 5 mL acetic acid solution to weigh boat “C”.
8. Develop a hypothesis as to what the pH of the contents of each container is. Record your hypothesis in
Post-Lab Question 1.
9. Use the litmus test strips to determine if the substances in containers A - C are acidic, basic, or neutral.
This is accomplished by briefly dipping an unused strip of the litmus paper in each of the weigh boats.
Record your color results in Table 2. Record results immediately after dipping. The litmus paper may become darker as it dries.
10. Pipette 1 mL of acetic acid from weigh boat “C” into beaker “B”. Ensure the transfer pipette does not touch the solution in beaker “B”.
11. Gently pipette the solution in beaker B with its pipette to mix.
12. Test the pH of beaker “B” using new litmus paper and immediately record your result in Table 3.
13. Repeat Steps 10 - 12 four more times until all the acetic acid has been added to beaker “B”.
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Experiment 1: Neutralization of Acids and Bases
In this experiment, you will learn how to properly neutralize and dispose of acidic and basic solutions.
Materials
5 mL 4.5% Acetic Acid (vinegar), C2H4O2
2 Pipettes
(1) 250 mL Beaker
0.5 g Sodium Bicarbonate (baking soda), NaHCO3
(1) 10 mL Graduated Cylinder
2 Weigh Boats
(1) 100 mL Graduated Cylinder
*Water
8 Litmus Test Strips (Neutral)
Permanent Marker
*You Must Provide
Procedure
1. Use the permanent marker to label two of the weigh boats as A and C.
2. Use the permanent marker to label a 250 mL beaker B.
3. Set the containers in the order A, B, and C.
4. Measure and pour approximately 5 mL of water into weigh boat “A”.
5. Add 0.5 g sodium bicarbonate to beaker “B”.
6. Use the 100 mL graduated cylinder to measure and pour 100 mL of water into beaker “B”. Gently pipette the solution up and down until the sodium bicarbonate is fully dissolved in the water. Leave this pipette in beaker B to mix the solution in future steps.
7. Use the 10 mL graduate cylinder to measure and pour 5 mL acetic acid solution to weigh boat “C”.
8. Develop a hypothesis as to what the pH of the contents of each container is. Record your hypothesis in
Post-Lab Question 1.
9. Use the litmus test strips to determine if the substances in containers A - C are acidic, basic, or neutral.
This is accomplished by briefly dipping an unused strip of the litmus paper in each of the weigh boats.
Record your color results in Table 2. Record results immediately after dipping. The litmus paper may become darker as it dries.
10. Pipette 1 mL of acetic acid from weigh boat “C” into beaker “B”. Ensure the transfer pipette does not touch the solution in beaker “B”.
11. Gently pipette the solution in beaker B with its pipette to mix.
12. Test the pH of beaker “B” using new litmus paper and immediately record your result in Table 3.
13. Repeat Steps 10 - 12 four more times until all the acetic acid has been added to beaker “B”.
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