Catalase enzyme study flow chart
A Quantitative Enzyme Study: CATALASE
FlowChart
Purpose: Measure the rate of enzyme activity in different conditions
Procedure:
A. Extraction of Catalase
1. Peel potato
2. Cut into cubes
3. Mass 50g
4. Measure out 50ml of cold distilled water in blender
5. Add crushed ice into blender (small amount)
6. Add the potato cubes into the blender
7. Turn on blender on high for 30 seconds
8. Prepare an ice bath
- FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH –
9. Filter potato extract
10. Place extract into a 100ml graduated cylinder
11. Add cold distilled water to the cylinder until volume equals 100ml
12. Extract to be labeled 100 units of enzyme per ml
B. Effect of Catalase Concentration
1. Obtain four 50ml beakers
2. Llabel beakers 100, 75, 50, and 0 units/ per ml
3. Prepare 40ml of enzme for each of the concentrations
40ml of original enzyme + 0ml cold distilled water =100units/ml
30ml of original enzyme + 10ml cold distilled water=75units/ml
20ml of original enzyme + 20ml cold distilled water=50units/ml
0ml of original enzyme + 40ml cold distilled water=0units/ml
4. Save undiluted enzyme for parts C-E
5. Place concentrations in ice bath
6. Obtain an additional four 50ml beakers
7. Label beakers 100, 75, 50, and 0 units/per ml
8. Measure 40ml of a 1% hydrogen peroxide solution into each of the four beakers
9. Using forceps drop a 2.1 cm glass fiber filter disc to ½ its diameter in the catalase solution (start with 100units/ml)
10. Leave inside solution for 5 seconds
11. Remove disk and place on a paper towel for 10 seconds
12. 1st person will obtain a stopwatch
13. 2nd person will drop the disc into the concentrations corresponding substrate solution
14. 1st person starts stopwatch when the disk touches the surface of the solution to the time it takes to reach the surface
15. Rate of enzyme activity equals one over the time in seconds the disc took to reach the surface. (r)= 1/(t sec)
16.
FlowChart
Purpose: Measure the rate of enzyme activity in different conditions
Procedure:
A. Extraction of Catalase
1. Peel potato
2. Cut into cubes
3. Mass 50g
4. Measure out 50ml of cold distilled water in blender
5. Add crushed ice into blender (small amount)
6. Add the potato cubes into the blender
7. Turn on blender on high for 30 seconds
8. Prepare an ice bath
- FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH –
9. Filter potato extract
10. Place extract into a 100ml graduated cylinder
11. Add cold distilled water to the cylinder until volume equals 100ml
12. Extract to be labeled 100 units of enzyme per ml
B. Effect of Catalase Concentration
1. Obtain four 50ml beakers
2. Llabel beakers 100, 75, 50, and 0 units/ per ml
3. Prepare 40ml of enzme for each of the concentrations
40ml of original enzyme + 0ml cold distilled water =100units/ml
30ml of original enzyme + 10ml cold distilled water=75units/ml
20ml of original enzyme + 20ml cold distilled water=50units/ml
0ml of original enzyme + 40ml cold distilled water=0units/ml
4. Save undiluted enzyme for parts C-E
5. Place concentrations in ice bath
6. Obtain an additional four 50ml beakers
7. Label beakers 100, 75, 50, and 0 units/per ml
8. Measure 40ml of a 1% hydrogen peroxide solution into each of the four beakers
9. Using forceps drop a 2.1 cm glass fiber filter disc to ½ its diameter in the catalase solution (start with 100units/ml)
10. Leave inside solution for 5 seconds
11. Remove disk and place on a paper towel for 10 seconds
12. 1st person will obtain a stopwatch
13. 2nd person will drop the disc into the concentrations corresponding substrate solution
14. 1st person starts stopwatch when the disk touches the surface of the solution to the time it takes to reach the surface
15. Rate of enzyme activity equals one over the time in seconds the disc took to reach the surface. (r)= 1/(t sec)
16.