In the second separation of this experiment, the Erlenmeyer flask with the first filtrate was put into a beaker so the filtrate was in the beaker. The beaker was then heated on a hotplate until near boiling. After the whey protein had precipitated, a vacuum filter was used to filter out the whey. These proteins were then air-dried. A qualitative test was done to indicate that the sample extracted was the protein whey. Again, a small test tube was used and 2 mL of protein test solution was put in the tube. Five drops of 10% NaOH and 2 drops of 0.5% CuSO4 were added to this tube. If a purple color showed, it would indicate there was protein in the test tube.…
In order to experiment with distinct components of a mixture, they must be first separated so they can be observed individually. This is accomplished in this lab by the technique called thin layer chromatography (TLC). TLC involves a stationary phase, which the TLC plates as well as a mobile phase, which could be one of two solvents used: ethanol-acetone for TLC. Dyes in a sample separate consequently because of their unique polarities. As a result, nonpolar substances travel further than polar substances in this process. The separation of a mixture into its components by TLC transpires because the distinctive components of a mixture interact to different extents with the stationary and mobile phases.…
I followed the preparation and the extraction procedure according to the lab manual (Biology Department, 2014).…
In this experiment we also used a chemical method known as extraction. This process of separation is used when only one component of the mixture is soluble in a specific solvent. In our case we had to use hydrochloric acid (HCl).Extractions, is a way to separate a desired substance when it is mixed with others. The mixture is brought into contact with a solvent in which the substance of interest is soluble, but the other substances present are insoluble. And below is a…
3. You purified protein X via affinity chromatography (no diafiltration step performed) and ran an SDS-PAGE gel of the sample with a set of controls. Below is the result of your SDS-PAGE analysis.…
Roe, S. (2001). _Protein Purification Techniques: A Practical Approach_ (2nd Ed.). United Kingdom: Oxford University Press…
After completing the first portion, the secondary portion requires that one determine the protein content by measuring absorbance and various protein concentration values. There are, however, to unknown proteins with the given codes U1-K and U2-Q. By utilizing a standard curve one is able to obtain the unknown protein concentrations of BSA while also converting the absorbance readings of the unknown proteins to concentration values.…
one labeled 10% for sample A and at lab table #2 there would be one dish labeled 20%…
Canada is a country built on immigration, mainly British at first, but from all over the world afterwards. Chinese immigration to Canada dates back to the period of the gold rush when they were recruited to mine. In the 1880's, the Chinese population increased after Canada joined the confederation and extra labour was required to build a cross-country railway. Due to the poor economy in China, many Chinese were willing to migrate and work for low wages. The Canadian government seized this opportunity and allowed the companies working on the Canadian Pacific Railway (CPR) to import Chinese immigrants. With a drive for completion a significant number of Chinese were recruited. In order to control this increase in immigration, the Canadian government implemented a policy imposing a head-tax on Chinese immigrants entering Canada. The head-tax was a discriminatory piece of legislation, which was enacted by the government due to public pressure, and which led to severe discrimination of the Chinese people, thus violating present day human rights codes.…
Thin layer chromatography can be used as a physical method to segregate compounds from natural sources. E.g. Spinach leaves are visibly green, but consist of a variety of components that have more colour than others. This experimental procedure uses compounds from spinach leaves that are exposed to chromatography, TLC plate to indicate the different pigments within the spinach extract.…
Note: This sample test is just a sample for you to see what a real exam looks like.…
Statistical data dates back to as early as Ancient Greek time, where it was introduced by John Graunt, William Petty and Pascal in the 16th century. It was then re-introduced by Gottfriend Achenwall in the 17th century. This was a very exciting time for scientists, astronomers and physicists alike as it raised the confidence and knowing that the laws of nature were not of divine intervention. As the years went on, new mathematical discoveries were made such as census data, economy, mortality demographics, and the International Statistical Congresses, which all led to changing its name to “statistics”.…
The preparation of the extraction included the use of a scale to weigh samples, the operation of a pipette, and finally setting up and reading the results of the spectrophotometer.…
A person is being wheeled right through the door; the whole nursing staff head’s whip towards the cart. A doctor is on scene in less than a couple seconds writing all the symptoms the patient is showing; he immediately enters the data right into a computer system called RIP. This systems then displays the odds of the patient surviving. If the percent of death is 95 or higher all progress on the patient ceases and medical staff returns back to their stations. In the article “Medical Technology and Ethical Issues” by William E. Thompson and Joseph V. Hickey never discuss the accuracy of the computer system. When there is an error a person’s life is lost; a computer system should not be able to states whether someone should be given treatment or just die. If there is a five percent chance for someone may live then the risk should be made by the medical staff to operate. Another problem “Medical Technology and Ethical Issues” never elaborate on is what RIP is in reference to. Is it referring to a company name or is it simply an acronym for “Rest in Peace.” Technology is advancing in every aspect, but the reliance of it should not. Doctors should refer to RIP when needing help on which steps to take for treatment but not give up when the system suggests no more treatment.…
“Greed is a bottomless pit which exhausts the person in an endless effort to satisfy the need without ever reaching satisfaction.” -Erich Fromm. Greed is an underlying theme that repeatedly takes form throughout F. Scott Fitzgerald’s novels, it eats up and consumes his characters to the point of their deterioration. They all yearn for an outcome that they will never get, however they feel that the world owes whatever it is that they seek to them. Fitzgerald uses his characters to criticize the upper class's greed, arguing that if someone achieves wealth, or is raised wealthy then it will lead to a feeling of entitlement. People who live affluently feel entitled to everything, especially love and money which results in failure and decline of…