Lab 1 again
Enzyme Kinetics and Protein Determination: How Enzyme Catalase Concentration Affects Reaction Rate and Determining the Identity of Unknown Proteins through Absorbance
By: Alexander Mak
7238991
Partner: Yasmin Ismail
BIO2137 Section A7
Corrector:
Chieu Anh Ta
September 18, 2014
Introduction:
The first lab's primary objective is to observe the different reactions rates amongst the five different catalse concentrations of parsnip. The rate at which the enzyme catalyzes increases in relation to the increase of concentration within the test tubes. By increasing the concentration per total volume, the reaction ultimately increases the amount of contents reacting per second while decreasing the overall activation energy. The procedure involves soaking filter papers in the catalase solution and time the reaction as it makes contact with hydrogen peroxide (H2O2 (aq).
After completing the first portion, the secondary portion requires that one determine the protein content by measuring absorbance and various protein concentration values. There are, however, to unknown proteins with the given codes U1-K and U2-Q. By utilizing a standard curve one is able to obtain the unknown protein concentrations of BSA while also converting the absorbance readings of the unknown proteins to concentration values.
Figure 1: The reaction rates of enzyme-catalysed reactions of hydrogen peroxide into diluted water depending of the varying percentage concentrations of catalase. Mean rates (±SE) of 5 separate experiments (n=5) are shown. Line of best fit (y = 0.0002x + 0.0038 R² = 0.9154) is presented using the trend line function in Excel.
Figure 2: Absorbance readings of different concentrations of BSA. Individual data is presented of one group is presented (n=1). Line of best fit (y = 0.2091x - 0.215 R² = 0.9527) is presented using the trend line function in Excel.
Results:
Part 1 Figure 1, the graph illustrated previously, demonstrates a
By: Alexander Mak
7238991
Partner: Yasmin Ismail
BIO2137 Section A7
Corrector:
Chieu Anh Ta
September 18, 2014
Introduction:
The first lab's primary objective is to observe the different reactions rates amongst the five different catalse concentrations of parsnip. The rate at which the enzyme catalyzes increases in relation to the increase of concentration within the test tubes. By increasing the concentration per total volume, the reaction ultimately increases the amount of contents reacting per second while decreasing the overall activation energy. The procedure involves soaking filter papers in the catalase solution and time the reaction as it makes contact with hydrogen peroxide (H2O2 (aq).
After completing the first portion, the secondary portion requires that one determine the protein content by measuring absorbance and various protein concentration values. There are, however, to unknown proteins with the given codes U1-K and U2-Q. By utilizing a standard curve one is able to obtain the unknown protein concentrations of BSA while also converting the absorbance readings of the unknown proteins to concentration values.
Figure 1: The reaction rates of enzyme-catalysed reactions of hydrogen peroxide into diluted water depending of the varying percentage concentrations of catalase. Mean rates (±SE) of 5 separate experiments (n=5) are shown. Line of best fit (y = 0.0002x + 0.0038 R² = 0.9154) is presented using the trend line function in Excel.
Figure 2: Absorbance readings of different concentrations of BSA. Individual data is presented of one group is presented (n=1). Line of best fit (y = 0.2091x - 0.215 R² = 0.9527) is presented using the trend line function in Excel.
Results:
Part 1 Figure 1, the graph illustrated previously, demonstrates a