Enzyme Tyrosinase Lab Report

The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount of light absorbed by the solution (absorbance) at various wavelengths.
The preparation of the extraction included the use of a scale to weigh samples, the operation of a pipette, and finally setting up and reading the results of the spectrophotometer.