Read section II of chapter 26 in your lecture textbook for background on chemical antimicrobial control (for background)—pages 762-763.…
Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorbed substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge, alterations or changes can be detected in the substance.…
The purpose of this laboratory investigation is to observe how chromatography can be used to separate mixtures of a solution.…
Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation, you can observe the different colors that make up a particular color of magic marker ink.…
Tryptophane is the largest amino acid. Also two larger amino acids are Alanine and Serine. The largest Rf is Tryptophane. The polarity is like the Serine but the Alanine is nonpolar.…
Discuss at least two of the chemical methods used to raise latent prints and advise your classmates on what piece of evidence you would process that way. Be sure to choose a different piece of evidence from your classmates…
3) Paper Chromatography Lab – Completed Procedure / Observations, questions, and a written description of most important concept that you learned in the discussion section.…
Paper chromatography is a logical technique used to separate works of a solution. Three examples of how we apply this technique to real-life would be: contaneminants in water, separation of plant pigmentation, and analysis of narcotics.…
(As The Editors Of Encyclopædia Britannica say “Paper chromatography, technique for separating dissolved chemical substances by taking advantage of their different rates of migration across sheets of paper. The method consists of applying the test solution or sample as a spot near one corner of a sheet of filter paper. The paper is initially impregnated with some suitable solvent to create a stationary liquid phase. An edge of the paper close to the spot is then immersed in another solvent in which the components of the mixture are soluble in varying degrees. The solvent penetrates the paper by capillary action and, in passing over the sample spot, carries along with it the various components of the sample.”) they explain what and how paper chromatography is used for. They all have to do with colors. Having to do with finding the real dyes in colors. (As Science Buddies explain “What color is black ink? Sometimes things are not just what we think they are. Often things can be broken down into component parts. One way to find out it to use a common chemistry technique called chromatography.”) This quote explains that there can be many object in just one object, just like paper chromatography. The paper chromatography experiment it tested to see what kind of dyes are just in one…
Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance, followed by chlorophyll a. Chlorophyll b is the most polar; therefore, it travels the shortest distance. The separated pigments on the chromatography paper can be eluted in acetone and absorbance spectrum is determined using spectrophotometer. Spectrophotometer produces a graph of the absorbance spectrum which shows the wavelengths (in nm) that are absorbed by the pigment and that are reflected. The reflected wavelength of chlorophylls a and b reflect the wavelengths of l light that are associated with green, which gives them their color. Beta carotene reflects the wavelengths that are associated with orange color, and that explains its color.…
In this experiment, there was one specific error, which was not holding the cup rim directly onto the middle of the paper. Instead, the paper was soaked by the water. This affected the results, because the tests weren’t done exactly the same, so therefore the results couldn’t be comparable. I can reduce this error, by making sure that the paper was directly onto the cup’s rim, without the water touching the paper…
During the crayon making process, I learned not to make assumptions or inferences until we finished collecting all of the data and observations. While the wax and dye was entirely melted, we observed that the liquid had turned a grey and transparent color. While this had led us to believe the crayon would not have a bright or solid color, the hardened crayon was a bright and consistent blue. We learned that through observation, we were able to make inferences about the consistency and strength of the color after the crayon hardened. We observed that our second crayon was more transparent in liquid form, and that it was less consistent when hard. In contrast, our first crayon was more opaque when in liquid form, hardening to become a consistent and solid color. This allowed us to make inferences about how the transparency of the crayon in liquid form allowed us to project the strength of the color once the hardening process was completed. This is important for a couple of reasons. First, it is important to recognize trends when doing experiments, this allowed us to refine our formulas when preparing for day 2. Second, it allowed us to learn how to apply our observations, something that will certainly be important moving forward as a learner and…
The process of making a spinach smoothie is quite easy, if one allows themselves time for preparation. There are several steps involved in making a spinach smoothie. Washing and cleaning of the vegetables, portioning the correct amount of ingredients, and proper blending are all important steps in the making of a spinach smoothie.…
Many people look up to others as a source of admiration. Celebrities are usually the main targets of idolization because of their lifestyles, careers, struggles and successes. Celebrities are portrayed in the media in ways that make common people want to be like them and do what they do. Celebrities live amazing lives from what we see. They accumulate wealth and fame which draws the attention of people. But for some people like myself, I admire celebrities because they are a source of hope. At the age of twelve I sat at my kitchen table flipping through a gossip magazine (I cannot remember which magazine probably Us Weekly or Star). I flipped the pages briefly reading any articles, but not paying much attention to them. I eventually stopped when I saw Paris Hilton. She was so beautiful with her blonde hair and blue eyes. I can remember it like it was yesterday: her hair was in a bob and she was wearing a lavender dress. At that time I did not know that I was going to become her number one fan, but no one ever does. Over the past six years I have been a fan of Paris Hilton. I was not always her number one fan, but as time progressed, I went from an ordinary fan to extraordinary number one fan. Following these steps was very successful for me and can be very vital for you. As long as you are fascinated by a celebrity and determined to become his or her number one fan, to act in accordance with these steps will be easy. When I became a super fan there was no one that helped me, but luckily I have compiled these guidelines to make it easier for you. There are a few guidelines that I have set up to help assist you as you begin your journey to becoming a celebrity’s number one fan.…
Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray.…