Fresh Parsley And Spinach Research
Research Question: What are the percent concentrations of Fe3+ ions by mass in fresh parsley and spinach leaves?
Scientific Concept Colorimeter measures the absorbance of light by the chemical substances at a certain wavelength. By measuring the absorbance, the concentration of a chemical substance can be determined. For the calibration of the colorimeter, different concentrations of a standard solution and a control (distilled water) are prepared in cuvettes. These cuvettes are put into the colorimeter one by one to calibrate it. After the colorimeter is calibrated, solutions of the substances which their concentrations are wanted to be determined, are put into cuvettes and their concentrations can be determined by this process (Dubey 209). …show more content…
Put the crucible on the pipe-stem triangle.
4. Light the Bunsen burner, turn to blue flame and heat the crucible until the parsley burns completely.
5. Take the crucible from the pipe-stem triangle and leave it to cool down.
6. Transfer the ash of parsley into a test tube by using a stirring rod.
7. Measure 5 mL distilled water by using a graduated cylinder with a dropper and add distilled water into the test tube, stir it with a stirring rod.
8. Put an empty test tube into a test tube holder.
9. Put filter paper into a funnel and put the funnel into the test tube in the test tube holder.
10. Filter the distilled water-ash mixture.
11. Put 5 mL KSCN solution into the test tube that the filtrate is in by using a …show more content…
Transfer the water-ash-KSCN mixture into a cuvette by using a dropper.
13. Repeat steps 1-12 for spinach leaf.
Using the colorimeter:
1. Connect the colorimeter to your computer.
2. Adjust the absorbance of the colorimeter to 450nm.
3. Approximately 15 minutes after you have prepared your standard solutions, start measuring the absorbance of the standard solutions by starting from the most dilute standard solution to obtain the calibration curve.
4. Measure the absorbance of parsley and spinach samples.
5. Calculate the amount of Fe3+ ions in parsley and spinach samples. Works Cited
Brooks, David W. “Simple Iron in Food Determination.” Web. 6 Dec. 2017.
"Determination of Iron by Thiocyanate Colorimetry." Canterbury.ac.nz. 2017. Web. 6 Dec. 2017.
Dubey, R. C. Advanced Biotechnology: For B Sc and M Sc Students of Biotechnology and Other
Biological Sciences. S Chand, 2014. Print.
“Iron.” University of Maryland Medical Center. 23 June 2015. Web. 27 Dec. 2017.
National Research Council. Diet and Health: Implications for Reducing Chronic Disease Risk.
1989. Print.
Oborn, Eugene T. Iron Content of Selected Water and Land Plants. United States Government
Printing Office, 1960.
Scientific Concept Colorimeter measures the absorbance of light by the chemical substances at a certain wavelength. By measuring the absorbance, the concentration of a chemical substance can be determined. For the calibration of the colorimeter, different concentrations of a standard solution and a control (distilled water) are prepared in cuvettes. These cuvettes are put into the colorimeter one by one to calibrate it. After the colorimeter is calibrated, solutions of the substances which their concentrations are wanted to be determined, are put into cuvettes and their concentrations can be determined by this process (Dubey 209). …show more content…
Put the crucible on the pipe-stem triangle.
4. Light the Bunsen burner, turn to blue flame and heat the crucible until the parsley burns completely.
5. Take the crucible from the pipe-stem triangle and leave it to cool down.
6. Transfer the ash of parsley into a test tube by using a stirring rod.
7. Measure 5 mL distilled water by using a graduated cylinder with a dropper and add distilled water into the test tube, stir it with a stirring rod.
8. Put an empty test tube into a test tube holder.
9. Put filter paper into a funnel and put the funnel into the test tube in the test tube holder.
10. Filter the distilled water-ash mixture.
11. Put 5 mL KSCN solution into the test tube that the filtrate is in by using a …show more content…
Transfer the water-ash-KSCN mixture into a cuvette by using a dropper.
13. Repeat steps 1-12 for spinach leaf.
Using the colorimeter:
1. Connect the colorimeter to your computer.
2. Adjust the absorbance of the colorimeter to 450nm.
3. Approximately 15 minutes after you have prepared your standard solutions, start measuring the absorbance of the standard solutions by starting from the most dilute standard solution to obtain the calibration curve.
4. Measure the absorbance of parsley and spinach samples.
5. Calculate the amount of Fe3+ ions in parsley and spinach samples. Works Cited
Brooks, David W. “Simple Iron in Food Determination.” Web. 6 Dec. 2017.
"Determination of Iron by Thiocyanate Colorimetry." Canterbury.ac.nz. 2017. Web. 6 Dec. 2017.
Dubey, R. C. Advanced Biotechnology: For B Sc and M Sc Students of Biotechnology and Other
Biological Sciences. S Chand, 2014. Print.
“Iron.” University of Maryland Medical Center. 23 June 2015. Web. 27 Dec. 2017.
National Research Council. Diet and Health: Implications for Reducing Chronic Disease Risk.
1989. Print.
Oborn, Eugene T. Iron Content of Selected Water and Land Plants. United States Government
Printing Office, 1960.