Gel Electrophoresis Synthesis Lab Report
Results
These are the images taken of the gel after electrophoresis.
Gel 1
Gel 2 Lane 7. This is Maddie’s (MCB) sample. Gel 2 Lane 6. This is Madi’s (MN) sample. From our sample of the gel electrophoresis, both Madi and me are homozygous positive (+/+) for the Alu gene. This can be determined by looking at the ladder and comparing our sample to it, to find out if we are homozygous or heterozygous.
Discussion For this lab, DNA from our cheek cells were separated through PCR, and singled out through gel electrophoresis. The process of the PCR is complex and contains different components and reactions. The first main step of PCR is denaturing. The temperature at which denaturing occurs is at 94°C. This first step separates the double stranded DNA template into two separate one stranded molecules. The second main step in PCR is Annealing. This step occurs at a temperature of 60°C. …show more content…
This was determined by using the ladder as a reference, and using past gel images as references as well. During our experiment, we tried our best to not contaminate any of our DNA, but that doesn't mean that it’s not impossible. Sources of contamination for our DNA could be other foreign DNA accidentally mixing with ours, for example, a skin cell could possibly get into the solution, which would throw off our results. Also, if gloves hadn't been worn, that would also mess with the results of the experiment. Mostly, we had to be careful of not mixing any other DNA with our cheek cell DNA. Once our experiment was conducted, we saw our gel image in the lab. The classes DNA was colored yellow and the ladder was blue and purple. Our final gel image was different than the one we observed in lab because a dye had been applied to the DNA so that it would show clearly where the DNA had moved after electrophoresis when a UV light was
These are the images taken of the gel after electrophoresis.
Gel 1
Gel 2 Lane 7. This is Maddie’s (MCB) sample. Gel 2 Lane 6. This is Madi’s (MN) sample. From our sample of the gel electrophoresis, both Madi and me are homozygous positive (+/+) for the Alu gene. This can be determined by looking at the ladder and comparing our sample to it, to find out if we are homozygous or heterozygous.
Discussion For this lab, DNA from our cheek cells were separated through PCR, and singled out through gel electrophoresis. The process of the PCR is complex and contains different components and reactions. The first main step of PCR is denaturing. The temperature at which denaturing occurs is at 94°C. This first step separates the double stranded DNA template into two separate one stranded molecules. The second main step in PCR is Annealing. This step occurs at a temperature of 60°C. …show more content…
This was determined by using the ladder as a reference, and using past gel images as references as well. During our experiment, we tried our best to not contaminate any of our DNA, but that doesn't mean that it’s not impossible. Sources of contamination for our DNA could be other foreign DNA accidentally mixing with ours, for example, a skin cell could possibly get into the solution, which would throw off our results. Also, if gloves hadn't been worn, that would also mess with the results of the experiment. Mostly, we had to be careful of not mixing any other DNA with our cheek cell DNA. Once our experiment was conducted, we saw our gel image in the lab. The classes DNA was colored yellow and the ladder was blue and purple. Our final gel image was different than the one we observed in lab because a dye had been applied to the DNA so that it would show clearly where the DNA had moved after electrophoresis when a UV light was