Genetically Modified Foods Lab Report
Abstract: The purpose of this lab experiment was to discover genetically modified organisms. We analyzed our results and found that our sample was GMO positive. These results were attained through polymerase chain reaction and agarose gel electrophoresis. We came to the conclusion that the presence of a band in lane 4 confirms that there are indeed genetically modified organisms.
Introduction: Overall, the main purpose of this experiment was to conduct gel electrophoresis combined with PCR in order to determine the presence of genetically modified organisms. A GMO is a genetically modified organism. The use of pesticides and herbicides lead to GMOs being an alternative to these products. GMOs are heavily used in the United States but frowned …show more content…
To do this we removed .5 g of agarose and added it in 25 ml of the buffer solution, heated it in the microwave at one minute intervals until the solution was clear, and then placed the bottled in a 65°C water bath until the temperature was balanced. Once it was cooled, we poured the molten gel into the casting tray with the gel comb already set in stone. We then let it solidify for 15-20 minutes. After it had solidified, we transferred it into the electrophoresis chamber. We loaded the PCR products by placing the tubes into the pulse-spin for 2-3 seconds, then adding 10 µl of the Orange-G dye to each tube, and then pulse-spinning them again for 2-3 seconds. The figure below shows how much of the sample was dispersed into each well. (Figure …show more content…
Looking at lane 2, we can see it did not accumulate a band because there is no GMO indicated. In lane 3 there is a presence of a band containing again 455 bp. Furthermore, lane 4 is a GMO positive consisting of 200 bp. Once again, in lane 5 there is 455 bp. Lane 6 shows that there is 200 bp. Ultimately, lane 7 is our ladder which consists of 1,000, 700, 500, 200, and 100 bp. Primer dimers are the faint bands that appear as markers in the gel. The process of GMO detection through PCR and gel electrophoresis consists of a staining dye. This dye carries forward and reverse primers that bind to specific sequence of DNA. We use the SYBER safe dye because it is formulated to be less hazardous than ethidium-bromide. The SYBER safe dye binds to the DNA and bands can then be visualized from UV light. The lanes that show proper PCR and DNA isolation are lanes 1, 3,4,5, and 6 because they are
Introduction: Overall, the main purpose of this experiment was to conduct gel electrophoresis combined with PCR in order to determine the presence of genetically modified organisms. A GMO is a genetically modified organism. The use of pesticides and herbicides lead to GMOs being an alternative to these products. GMOs are heavily used in the United States but frowned …show more content…
To do this we removed .5 g of agarose and added it in 25 ml of the buffer solution, heated it in the microwave at one minute intervals until the solution was clear, and then placed the bottled in a 65°C water bath until the temperature was balanced. Once it was cooled, we poured the molten gel into the casting tray with the gel comb already set in stone. We then let it solidify for 15-20 minutes. After it had solidified, we transferred it into the electrophoresis chamber. We loaded the PCR products by placing the tubes into the pulse-spin for 2-3 seconds, then adding 10 µl of the Orange-G dye to each tube, and then pulse-spinning them again for 2-3 seconds. The figure below shows how much of the sample was dispersed into each well. (Figure …show more content…
Looking at lane 2, we can see it did not accumulate a band because there is no GMO indicated. In lane 3 there is a presence of a band containing again 455 bp. Furthermore, lane 4 is a GMO positive consisting of 200 bp. Once again, in lane 5 there is 455 bp. Lane 6 shows that there is 200 bp. Ultimately, lane 7 is our ladder which consists of 1,000, 700, 500, 200, and 100 bp. Primer dimers are the faint bands that appear as markers in the gel. The process of GMO detection through PCR and gel electrophoresis consists of a staining dye. This dye carries forward and reverse primers that bind to specific sequence of DNA. We use the SYBER safe dye because it is formulated to be less hazardous than ethidium-bromide. The SYBER safe dye binds to the DNA and bands can then be visualized from UV light. The lanes that show proper PCR and DNA isolation are lanes 1, 3,4,5, and 6 because they are