Strawberry DNA Extraction Lab Formal Write Up
DNA Extraction Lab 1/17/14
Purpose :
This lab was conducted in order to show and analyze the way DNA is extracted.
Hypothesis:
If the lab is conducted properly then we should be able to view a visible amount of DNA from the strawberry and detergent mixture.
Variables:
The independent variable in this experiment is the strawberry mixture while the dependent variable is the amount of DNA extracted.
Procedure-summary:
Place a strawberry in a plastic baggy filled with the detergent mix and crush the strawberry, mixing the pulp with the detergent mix thoroughly, pour the detergent mixture into the funnel. Let the liquid from the mixture drain into the beaker then add the ethanol to the mixture.
Data:
Observations-
The ethanol sat on top of the detergent due to its lighter density.
Bubbles started rising as soon as the ethanol was added durn turning the liquid cloudy.
The DNA grouped rapidly, taking no longer than two to three minuets total before slowing down and seeming to stop grouping.
The DNA itself looked like sputum or phlegm, and was easily extracted from the mixture and sticky to the touch.
When the DNA was extracted from the test tube and the mixture was stirred, more DNA started to collect at the top.
Analysis:
-As the strawberry is physically mashed into the detergent the cells are broken down and opened. The ethanol is else dense than the mixture and draws the now accessible DNA to the surface where it is viewable.
-In comparing the extraction of strawberry DNA compared to human DNA, given a sample of the same amount of cells, there would be more DNA extracted from the strawberry for it has eight sets of chromosomes while humans just have two sets.
-In real world situations DNA extraction would be used in something as complex as a murder investigation, in which DNA would have to be
Purpose :
This lab was conducted in order to show and analyze the way DNA is extracted.
Hypothesis:
If the lab is conducted properly then we should be able to view a visible amount of DNA from the strawberry and detergent mixture.
Variables:
The independent variable in this experiment is the strawberry mixture while the dependent variable is the amount of DNA extracted.
Procedure-summary:
Place a strawberry in a plastic baggy filled with the detergent mix and crush the strawberry, mixing the pulp with the detergent mix thoroughly, pour the detergent mixture into the funnel. Let the liquid from the mixture drain into the beaker then add the ethanol to the mixture.
Data:
Observations-
The ethanol sat on top of the detergent due to its lighter density.
Bubbles started rising as soon as the ethanol was added durn turning the liquid cloudy.
The DNA grouped rapidly, taking no longer than two to three minuets total before slowing down and seeming to stop grouping.
The DNA itself looked like sputum or phlegm, and was easily extracted from the mixture and sticky to the touch.
When the DNA was extracted from the test tube and the mixture was stirred, more DNA started to collect at the top.
Analysis:
-As the strawberry is physically mashed into the detergent the cells are broken down and opened. The ethanol is else dense than the mixture and draws the now accessible DNA to the surface where it is viewable.
-In comparing the extraction of strawberry DNA compared to human DNA, given a sample of the same amount of cells, there would be more DNA extracted from the strawberry for it has eight sets of chromosomes while humans just have two sets.
-In real world situations DNA extraction would be used in something as complex as a murder investigation, in which DNA would have to be