Preview

Glucose Effect on Diabetic and Non Diabetic Blood

Good Essays
Open Document
Open Document
879 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Glucose Effect on Diabetic and Non Diabetic Blood
BSC 120
Glucose Effect on Diabetic and Non Diabetic Blood
Abstract: This lab was developed to investigate blood glucose and diabetes. Diabetes is a lifelong chronic disease in which there are high levels of sugar in the blood (Diabetes). The spectrophotometer was applied to this lab to determine the absorbance of blood glucose in diabetic and non-diabetic blood samples. In order to prove this, tests were conducted by taking the blood samples at different times right before a meal was eaten then 30, 60, 90, and 120 minutes after the meal. The 6 test tubes had been placed in the spectrophotometer to measure the absorbance of blood glucose in the diabetic and non-diabetic blood. It was hypothesized that people with diabetes will absorb more light from the higher amount of glucose in their body measured by the spectrophotometer compared to non-diabetics being less absorbent from lack of glucose. The results will show the significant difference in the absorption level for diabetic and non-diabetic blood. The level of blood glucose will remain higher longer in diabetics than non-diabetics. This experiment could have been easily flawed by human error but collectively the hypothesis seemed to be correct in this experiment. Those with diabetes would absorb more light from the higher amount of glucose in their body compared to non-diabetics measured by the spectrophotometer.
Introduction: The purpose of this experiment was to determine the blood glucose of each sample of diabetic and non-diabetic blood samples. Diabetes occurs when an individual develops a dysfunction in insulin production and/or insulin action, which induces an inability to metabolize glucose carried within the blood stream (Boyer). This experiment tested how quickly blood glucose returns to the pre-meal value in both diabetic and non-diabetic blood samples. With this being said, it was hypothesized that people with diabetes will absorb more light from the higher amount of glucose in their body measured



Cited: Boyer, Brett “Comprehensive handbook of clinical health psychology.” (2007) Ch. 8. Google Scholar. Web 15 Oct. 2009. "Diabetes - PubMed Health." PubMed Health. Ed. David Zieve and David R. Eltz. U.S. National Library of Medicine, 31 Aug. 2011. Web. 14 Nov. 2011. <http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0002194/>. Weinstein, Susan “Table 7.1. Absorbance data for non-diabetic and diabetic “blood” samples during a 2 hour time period.” Marshall University BSC 120 Lab Manual (2009):81. Weinstein, Susan “Using Spectrophotometry to measure blood glucose.” Marshall University BSC 120 Lab Manual (2009):73.

You May Also Find These Documents Helpful

  • Powerful Essays

    1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other),…

    • 1262 Words
    • 10 Pages
    Powerful Essays
  • Satisfactory Essays

    Brent Good Unit 3 Lab

    • 1058 Words
    • 10 Pages

    1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other),…

    • 1058 Words
    • 10 Pages
    Satisfactory Essays
  • Satisfactory Essays

    the physio ex

    • 503 Words
    • 4 Pages

    2. The larger value obtained when more glucose carriers were present corresponds to an increase in the rate of glucose…

    • 503 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    Blood Sugar and Post Meal

    • 775 Words
    • 6 Pages

    2.When was plasma glucose concentration lowest? If values are similar for several time points, then give range of times when plasma glucose concentration was lowest. 3 hours post meal…

    • 775 Words
    • 6 Pages
    Good Essays
  • Good Essays

    This blood test done after every 3 months helps to determine how good patient is controlling his or her blood sugar levels( prevention research group, 2002) it has come to clear conclusion that new evidence based research is using AIC tests as a laboratory tool for diagnosing the patient with diabetes. ADAG uses mathematical equation that may be used in converting the AIC test results to estimated average glucose and they were successful in coming up with the ideas. This study of using AIC result could be expressed as an estimated average glucose using the mathematical equation. Based on ADAG current research, they come up with the ideas of comparing the AIC values for each patient, together with daily glucose reading for two days and in four times a day (Nathan, M. et al. (2008)…

    • 1027 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Ramchandani, Saadon, Journsay. (2010). Real-time glucose monitoring. American Journal of Nursing. 110(4), 60-63. Retrieved from http://ovidsp.tx.ovid.com.library.gcu.edu…

    • 863 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Because of the wide variety of risks diabetes puts a person under, it is important to study it and compare diabetic blood to non-diabetic blood in order to have a deeper understanding of what it is doing to the body. With the experiment I hypothesized that the diabetic blood would have a higher absorbance reading than the non-diabetic.…

    • 618 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    People, who support weight loss plans, believe that isolating the different causes of obesity and overweight will guarantee weight loss in a short amount of time. By isolating the different causes of weight gain, the supporters of the weight loss plans believe that losing weight will be made simpler for people, who would want to lose weight without devoting too much time for exercise and dieting. The causes of weight gain are determined by a person’s consumption of food and production of hormones. The supporters of weight loss plans feel that limiting the production of certain hormones through the regulation of food consumption can significantly reduce weight lose in comparison to exercise and dieting.…

    • 1233 Words
    • 4 Pages
    Powerful Essays
  • Good Essays

    Beta- carotene is an organic compound categorized as a terpenoid (Gutiérrez, & Gonzalez, 2010). Naturally, it is a predecessor of vitamin A, and found in several yellow fruits and vegetables. These organic nutrients play a vital role in aiding vision, skin growth, and bone development. In addition to these crucial roles, Beta-carotene may be of significant help in regulating the blood sugar in diabetic persons (Gutiérrez, & Gonzalez, 2010). Human bodies regulate blood sugar levels with the help of the hormone called insulin. In the absence or inadequate supply of this hormone, an individual is said to be diabetic. Diabetes may results in solemn conditions such as blindness, heart diseases, and stroke. Over the years, medical experts have found a link between Beta- carotene and diabetes (Gutiérrez, & Gonzalez, 2010). Through lab researches, scientists identified that beta-carotene compounds were significantly lower among diabetic patients (Gutiérrez, & Gonzalez, 2010).…

    • 946 Words
    • 4 Pages
    Good Essays
  • Good Essays

    For the OGTT, samples have been provided from 3 patients named (A, B, C). Moreover a random plasma glucose test was given to an additional patient (D) without fasting and without OGTT. The three patients (A, B, C) fasted for 12 hours before attending the clinic and on arrival an OGTT was given to all the three patients. The blood was then collected in fluoride/EDTA anticoagulant and centrifuged to separate out the blood cells. The samples are chosen as fasting (0) and 2 hour (2) for example B0 and B2 and can be used directly without dilution. But sample A2 needed to be diluted 1 + 2 with distilled water before use i.e. I ml of A2 was delivered into a clean LP4 tube by glass pipette followed by 2 ml of water. This diluted sample was used for the assay. Sample D was also prepared in the same way but without dilution. Duplicate tubes were prepared from 200 micro litres of all samples A to D.Standards were prepared from the…

    • 772 Words
    • 4 Pages
    Good Essays
  • Good Essays

    The purpose of the Variables in Glucose Tolerance lab was to examine the changes in blood sugar during glucose tolerance test conducted with test rats. We will be using three rats and perform the Oral Glucose Tolerance Test to measure the glucose levels in the rats blood. The oral glucose tolerance test is a laboratory method to check how the body breaks down (metabolizes) blood sugar. Glucose is the sugar that the body uses for energy. Patients with untreated diabetes have high blood glucose levels. Glucose tolerance tests are one of the tools used to diagnose diabetes. (Hypothysis)…

    • 1179 Words
    • 5 Pages
    Good Essays
  • Satisfactory Essays

    39. Sasaki T, Matsy S, Sonae A. Effect of acetic acid concentration on the colour reaction in the O-toluidine boric acid method for blood glucose estimation. Rinsho Kagaku. 1972;1:346–353.…

    • 2483 Words
    • 10 Pages
    Satisfactory Essays
  • Powerful Essays

    tio2

    • 2935 Words
    • 12 Pages

    high sensitivity to glucose in the range of 0 to 1.8 mM with a response time of 3 s and detection limit of…

    • 2935 Words
    • 12 Pages
    Powerful Essays
  • Good Essays

    To detect rats which have false low blood glucose level however they are diabetic. 13 nude rats undergo fasting for half a day (12h), then they were given a glucose solution of 2g/kg.…

    • 1061 Words
    • 5 Pages
    Good Essays