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How Does Bromelain Affect The Gelatine

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How Does Bromelain Affect The Gelatine
INVESTIGATION RESEARCH
Introduction
There is approximately 75,000 enzymes that exist in the human body. Enzymes are important for fast reactions that keep you alive, break down large molecules into smaller molecules and use small molecules to build large complex molecules like the enzymes that make DNA and enzymes also help cells to communicate with each other. I have decided to use not one but two enzymes to observe what happens to the different kind of enzymes when introduced to different PH (power of hydrogen) scales. the best way to test this was to put the enzyme bromelain in gelatine because bromelain affects gelatine. If added to the gelatine, the bromelain in pineapples will prevent the gelatine from setting. However, different circumstances
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Enzymes have many advantaged and disadvantages:
Advantages
• Enzymes speed up biochemical processes
• Enzymes are very specific when it comes to reaction they are catalysing and which substrate they are working on.
• They work in mild conditions which saves a lot of energy
• help to speed up digestion processes in the human body.
Disadvantages
• Easily denatured
• Enzymes are difficult to work with because substrate mixtures must be uncontaminated with other substances that might influence the reaction
• highly responsive to changes in physical and chemical conditions surrounding them. It is difficult to manipulate their working

Bromelain found in pineapple. Used to treat muscle injuries and as a digestive aid. It is a natural anticoagulant that works by breaking down the blood-clotting protein. The optimum temperature is 62 degrees Celsius, activity decreases (protein denatures) at 70 degrees Celsius. The optimum pH is 3.0 to 6.5 and when combined with its substrate, the activity is no longer susceptible to the effect of the pH . Bromelain is an enzyme is activated by a substrate, which fits into a small part of the
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On the graph the variables level out meaning they have reached the optimum activity and are starting to level out the alkaline raised quickly and levelled out at a solid state while the acid and neutral raised fairly slower and levelled out at a semi liquid state which tells us the enzyme bromalin was more active in the neutral and acid pH s because it broke the jelly down (biological catalysts) and was less active in the alkaline because it didn’t break the alkaline

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