How Heat Affects Lipase
Factors Affecting Enzyme Action
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Term 1 Biology
Nicole Goosen
Table of Contents Introduction: 3 Materials: 3 Method: 4 Risk assignment: 4 Risk: 4 Personal Protective Equipment: 4 Results: 5 Discussion: 5 Conclusion: 6
Introduction:
How is the human body able to digest the food that you eat? How quickly your body digests your food? This is because the human body contains enzymes that are the biological substance, a.k.a proteins, that act as catalysts and help complex reactions occur. There are many different enzymes that each have different specific functions. The enzymes include: diastase, pepsin, lipase, catalyse and urease. Lipase is an enzyme found in the digestive tract that catalyses the break down of fats into individual fatty acids that can be absorbed into the bloodstream. This enzyme will be used in this EEI. The purpose of this EEI is to test the rate in which lipase reacts to different variables such as temperature and pH. Enzymes are found in foods, epically raw foods such as meat, but cooking foods destroys any enzyme activity, including lipases. If the temperature changes then the pH levels will change. The optimum temperature is 37°, that is the average temperature inside the human body; the optimum pH level is 8.0. (Corporation, 2013) The purpose of this EEI and experiment is to calculate the rate in which the pH level of milk changes after it has been heated and the heated lipase is added. Four different temperature will be tested to create the most accrete results possible. When lipase is heated, it becomes a fatty acid that destroys itself, so when the lipase is heated and then added to the heated milk, it rate in which the pH level changes should be high and fast. If the lipase and milk is heated above its optimum temperature (42°) then combined, the pH level will change much quicker and the change in pH levels will be much more clear.
Materials: * Full
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Term 1 Biology
Nicole Goosen
Table of Contents Introduction: 3 Materials: 3 Method: 4 Risk assignment: 4 Risk: 4 Personal Protective Equipment: 4 Results: 5 Discussion: 5 Conclusion: 6
Introduction:
How is the human body able to digest the food that you eat? How quickly your body digests your food? This is because the human body contains enzymes that are the biological substance, a.k.a proteins, that act as catalysts and help complex reactions occur. There are many different enzymes that each have different specific functions. The enzymes include: diastase, pepsin, lipase, catalyse and urease. Lipase is an enzyme found in the digestive tract that catalyses the break down of fats into individual fatty acids that can be absorbed into the bloodstream. This enzyme will be used in this EEI. The purpose of this EEI is to test the rate in which lipase reacts to different variables such as temperature and pH. Enzymes are found in foods, epically raw foods such as meat, but cooking foods destroys any enzyme activity, including lipases. If the temperature changes then the pH levels will change. The optimum temperature is 37°, that is the average temperature inside the human body; the optimum pH level is 8.0. (Corporation, 2013) The purpose of this EEI and experiment is to calculate the rate in which the pH level of milk changes after it has been heated and the heated lipase is added. Four different temperature will be tested to create the most accrete results possible. When lipase is heated, it becomes a fatty acid that destroys itself, so when the lipase is heated and then added to the heated milk, it rate in which the pH level changes should be high and fast. If the lipase and milk is heated above its optimum temperature (42°) then combined, the pH level will change much quicker and the change in pH levels will be much more clear.
Materials: * Full
Bibliography: Worthington Biochemical Corporation ha put together many different papers on the effects of enzyme action into one paper. It was originally published in 1972 but was last updated in 2013. This article gives a lot of information on the effects of enzyme action that has been seen on many other articles, it shows to be reliable because of the many authors that have been credited. This is a reliable source because these website holds many other useful experiments and general articles.