How Does Caffeine Affect Zebrafish
Nicoleta Nedelcu
Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action
Observation
Caffeine is found in coffee, tea leaves, cocoa beans, and kola nuts. It is used in prescription and over the counter drugs. It increases blood pressure, mental alertness, and gastrointestinal motility. Cardiovascular development with caffeine and other small molecules can be studied relatively easy using zebrafish. The cardiovascular system is complete in two days after fertilization.
Hypothesis
Methods
1. 1-25 mm of caffeine was injected in the yolk sacs of embryos 2-3 days after fertilization.
2. Adult fish were kept in individual tanks with sponge filters, at 28.5oC, under a 14 hour light and 10 hour dark cycle, and …show more content…
Those embryos with 1 mm of caffeine immediately had decreased heart rates and it continued to decrease for 70 minutes, then the heart rate was stable for 100 minutes, and then it sharply dropped and stopped after 27 minutes. Those embryos with 4 mm f caffeine has similar starting reaction for the first 10 minutes, then the heart rate was stable for the next 61 minutes, then it stopped after 1 minute. Those embryos with 8 mm of caffeine had similar reactions but the heart rate stopped after 44 minutes. Those with 10 mm of caffeine ended at 20 minutes, those with 12 mm of caffeine ended at 16 minutes. And those with 25 mm of caffeine stopped at 14 …show more content…
Critique It doesn’t specify what the hypothesis is which is not good, but the procedures were very detailed. They had control groups for when they used the calcium, but it doesn’t say anything about a control group when they used the caffeine. I like that they used different concentrations of caffeine but I would have liked for them to record the heart rate for an embryo without caffeine. Even though they did not have as many control groups as I would have wanted they were able to prove that the increase in the concentration of caffeine results in decreased heart rate and early death.
Word Cite
Rana, Neha, et al. "Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action: an effective system for experiments in chemical biology." Zebrafish, vol. 7, no. 1, 2010, p. 69+. Academic OneFile, libproxy.gc.maricopa.edu/login?url=http://go.galegroup.com/ps/i.do?p=AONE&sw=w&u=mcc_glendale&v=2.1&it=r&id=GALE%7CA226047143&sid=summon&asid=21014119af311f6d02883e0c8148edf4. Accessed 4 Apr.
Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action
Observation
Caffeine is found in coffee, tea leaves, cocoa beans, and kola nuts. It is used in prescription and over the counter drugs. It increases blood pressure, mental alertness, and gastrointestinal motility. Cardiovascular development with caffeine and other small molecules can be studied relatively easy using zebrafish. The cardiovascular system is complete in two days after fertilization.
Hypothesis
Methods
1. 1-25 mm of caffeine was injected in the yolk sacs of embryos 2-3 days after fertilization.
2. Adult fish were kept in individual tanks with sponge filters, at 28.5oC, under a 14 hour light and 10 hour dark cycle, and …show more content…
Those embryos with 1 mm of caffeine immediately had decreased heart rates and it continued to decrease for 70 minutes, then the heart rate was stable for 100 minutes, and then it sharply dropped and stopped after 27 minutes. Those embryos with 4 mm f caffeine has similar starting reaction for the first 10 minutes, then the heart rate was stable for the next 61 minutes, then it stopped after 1 minute. Those embryos with 8 mm of caffeine had similar reactions but the heart rate stopped after 44 minutes. Those with 10 mm of caffeine ended at 20 minutes, those with 12 mm of caffeine ended at 16 minutes. And those with 25 mm of caffeine stopped at 14 …show more content…
Critique It doesn’t specify what the hypothesis is which is not good, but the procedures were very detailed. They had control groups for when they used the calcium, but it doesn’t say anything about a control group when they used the caffeine. I like that they used different concentrations of caffeine but I would have liked for them to record the heart rate for an embryo without caffeine. Even though they did not have as many control groups as I would have wanted they were able to prove that the increase in the concentration of caffeine results in decreased heart rate and early death.
Word Cite
Rana, Neha, et al. "Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action: an effective system for experiments in chemical biology." Zebrafish, vol. 7, no. 1, 2010, p. 69+. Academic OneFile, libproxy.gc.maricopa.edu/login?url=http://go.galegroup.com/ps/i.do?p=AONE&sw=w&u=mcc_glendale&v=2.1&it=r&id=GALE%7CA226047143&sid=summon&asid=21014119af311f6d02883e0c8148edf4. Accessed 4 Apr.