How Does Ph Affect The Rate Of Respiration In Yeast
Organisms through cellular respiration convert energy into ATP. They do this in three distinct
stages; glycolysis,the Krebs cycle and the electron transport chain. The Krebs cycle and electron
transport chain are both aerobic process and require oxygen. In certain conditions where oxygen
is not present or is low some organisms switch from cellular respiration into alcoholic or lactic
acid fermentation. Fermentation is an anaerobic, metabolic pathway used to oxidize nicotinamide
adenine dinucleotide (reduced) (NADH) into nicotinamide-adenine-dinucleotide (NAD+). As
with many other unicellular organisms, yeast ,cannot carry out cellular respiration and tends to
participate in fermentation and produces ethanol.
In has been suggested …show more content…
One can predict that the tube with the highest concentration
of yeast will produce the most carbon dioxide and will have the fastest rate of alcoholic
fermentation.
Results
Two separate experiments were conducted to determine the effect of yeast concentration and pH
level on the rate of fermentation. The first experiment involved the placement of different
concentrations of yeast, glucose, and distilled water in four different tubes. Pipette pumps were
placed on each tube to allow the solution to ferment, allowing carbon dioxide (CO2) bubbles.
Results were collected and data was obtained (Table 1). While there seemed to be little to no
production of carbon dioxide (CO2) in most of the tubes , the ones with higher concentrations of
yeasts produced minuscule amounts of carbon dioxide (Figure 1). The second experiment
involved the exact procedure but replaces the distilled water with a pH4 solution. Results were
obtained (Table 2). This experiment had CO2 bubbles in each tube at a very short time and this
indicates that it had a higher rate of …show more content…
However the tubes
with higher concentrations of yeast still produced a minuscule amount of CO2 unlike the ones
with lower concentrations.
Discussion
After reviewing the data collected one can perceive that the first experiment produced almost no
CO2 , this could mean that different concentrations of yeast does not play a significant role in
terms of fermentation or we had a major source of error in our part. The yeast solution used in
the lab could have surpassed its optimum temperature as it was taken in and out of the water bath
several times. Future investigations should consider the effect of temperature on the rate of
fermentation. This experiment could have been improved tremendously if the yeast was tested in
several pH solutions and not just one. A similar experiment conducted suggests that glucose
fractions had higher cell biomass production rate and therefore higher substrate consumption rate
and ethanol production rate compared to high xylose fractions(2006). Further
stages; glycolysis,the Krebs cycle and the electron transport chain. The Krebs cycle and electron
transport chain are both aerobic process and require oxygen. In certain conditions where oxygen
is not present or is low some organisms switch from cellular respiration into alcoholic or lactic
acid fermentation. Fermentation is an anaerobic, metabolic pathway used to oxidize nicotinamide
adenine dinucleotide (reduced) (NADH) into nicotinamide-adenine-dinucleotide (NAD+). As
with many other unicellular organisms, yeast ,cannot carry out cellular respiration and tends to
participate in fermentation and produces ethanol.
In has been suggested …show more content…
One can predict that the tube with the highest concentration
of yeast will produce the most carbon dioxide and will have the fastest rate of alcoholic
fermentation.
Results
Two separate experiments were conducted to determine the effect of yeast concentration and pH
level on the rate of fermentation. The first experiment involved the placement of different
concentrations of yeast, glucose, and distilled water in four different tubes. Pipette pumps were
placed on each tube to allow the solution to ferment, allowing carbon dioxide (CO2) bubbles.
Results were collected and data was obtained (Table 1). While there seemed to be little to no
production of carbon dioxide (CO2) in most of the tubes , the ones with higher concentrations of
yeasts produced minuscule amounts of carbon dioxide (Figure 1). The second experiment
involved the exact procedure but replaces the distilled water with a pH4 solution. Results were
obtained (Table 2). This experiment had CO2 bubbles in each tube at a very short time and this
indicates that it had a higher rate of …show more content…
However the tubes
with higher concentrations of yeast still produced a minuscule amount of CO2 unlike the ones
with lower concentrations.
Discussion
After reviewing the data collected one can perceive that the first experiment produced almost no
CO2 , this could mean that different concentrations of yeast does not play a significant role in
terms of fermentation or we had a major source of error in our part. The yeast solution used in
the lab could have surpassed its optimum temperature as it was taken in and out of the water bath
several times. Future investigations should consider the effect of temperature on the rate of
fermentation. This experiment could have been improved tremendously if the yeast was tested in
several pH solutions and not just one. A similar experiment conducted suggests that glucose
fractions had higher cell biomass production rate and therefore higher substrate consumption rate
and ethanol production rate compared to high xylose fractions(2006). Further