Lab 9: Stereochemistry And Polarimetry P
Hamdiya Mumade Partner: Isabella CHEM 253 TA: Pinky Chowdhury Oct 15, 2024 Lab 9: Stereochemistry and Polarimetry Purpose: To determine the concentration of three different vitamins by making a series of serial dilutions and using HPLC.
Theory: Here?s a theory section for your lab report on stereochemistry and polarimetry.
Theory Stereochemistry involves the study of how the spatial arrangement of atoms in a molecule influences its chemical and physical properties. Molecules with the same molecular formula but different arrangements of atoms are called isomers, which can be further classified into structural isomers and stereoisomers. Structural isomers have different bonding patterns, while stereoisomers have the same bonding but differ in the three-dimensional orientation of their atoms. Stereoisomers can be divided into two main types: enantiomers and diastereomers. Enantiomers are non-superimposable mirror images of each other, commonly found in compounds with at least one chiral carbon atom bonded to four distinct groups. These enantiomers have identical physical properties except when interacting with plane-polarized light or other chiral substances, making them distinguishable through their optical …show more content…
The unknown sample (X) showed an enantiomeric excess of 68.34% for the (R)-enantiomer, with an observed rotation of -30.25?, indicating levorotatory behavior. These results confirm the sample is not a racemic mixture, with a significant excess of (R)-limonene. While the experiment provided useful data, potential sources of error include slight misalignments in the polarimeter or sample impurities. Additionally, minor solvent loss during the filtration may impact the concentration and results. The TLC tests showed similar appearances for (S)- and (R)-limonene under UV light, suggesting that TLC is not ideal for resolving
Theory: Here?s a theory section for your lab report on stereochemistry and polarimetry.
Theory Stereochemistry involves the study of how the spatial arrangement of atoms in a molecule influences its chemical and physical properties. Molecules with the same molecular formula but different arrangements of atoms are called isomers, which can be further classified into structural isomers and stereoisomers. Structural isomers have different bonding patterns, while stereoisomers have the same bonding but differ in the three-dimensional orientation of their atoms. Stereoisomers can be divided into two main types: enantiomers and diastereomers. Enantiomers are non-superimposable mirror images of each other, commonly found in compounds with at least one chiral carbon atom bonded to four distinct groups. These enantiomers have identical physical properties except when interacting with plane-polarized light or other chiral substances, making them distinguishable through their optical …show more content…
The unknown sample (X) showed an enantiomeric excess of 68.34% for the (R)-enantiomer, with an observed rotation of -30.25?, indicating levorotatory behavior. These results confirm the sample is not a racemic mixture, with a significant excess of (R)-limonene. While the experiment provided useful data, potential sources of error include slight misalignments in the polarimeter or sample impurities. Additionally, minor solvent loss during the filtration may impact the concentration and results. The TLC tests showed similar appearances for (S)- and (R)-limonene under UV light, suggesting that TLC is not ideal for resolving