Lab Report about Simple Staining of Microbes
Abstract
This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion, different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify the basic shape and size of the microbe. In this experiment, the microbes E. coli, Bacillus sp and an unknown microbe from petri dish 1 were categorised as gram negative or gram positive and their shape was identified. These staining techniques are the correct way to identify the shape and size of microbes.
1.0 Introduction
Microbiology is the branch of biology that deals with microorganisms and their effect on other living organisms. Microbes are very small organisms which can only be viewed with the aid of microscope. Several groups of organisms that fit into this category are bacteria, cyanobacteria, fungi and protists. Within this group there are several species interesting to humans because of their ability to cause disease or their use in the food industry and microorganisms can be classified to unicellular and multicellular. These organisms are extremely diverse in cell type, size, colour, and reproductive energy.
Microbes can be classified by their cell type. All cells can be categorized either prokaryotic or eukaryotic and the primary difference between these two cell types is the presence of a membrane-bound nucleus. The experiment was carried out to use a bright field microscopy, prepare and observe bacterial slides and perform staining methods and explain the mechanism of the bacteria. In order to observe and investigate microbes we need to use microscope and bacterial Staining Techniques.
Microscope is the invaluable tool
This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion, different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify the basic shape and size of the microbe. In this experiment, the microbes E. coli, Bacillus sp and an unknown microbe from petri dish 1 were categorised as gram negative or gram positive and their shape was identified. These staining techniques are the correct way to identify the shape and size of microbes.
1.0 Introduction
Microbiology is the branch of biology that deals with microorganisms and their effect on other living organisms. Microbes are very small organisms which can only be viewed with the aid of microscope. Several groups of organisms that fit into this category are bacteria, cyanobacteria, fungi and protists. Within this group there are several species interesting to humans because of their ability to cause disease or their use in the food industry and microorganisms can be classified to unicellular and multicellular. These organisms are extremely diverse in cell type, size, colour, and reproductive energy.
Microbes can be classified by their cell type. All cells can be categorized either prokaryotic or eukaryotic and the primary difference between these two cell types is the presence of a membrane-bound nucleus. The experiment was carried out to use a bright field microscopy, prepare and observe bacterial slides and perform staining methods and explain the mechanism of the bacteria. In order to observe and investigate microbes we need to use microscope and bacterial Staining Techniques.
Microscope is the invaluable tool
References: 7) Bio-imaging, 2004, retrieved from: http://web.path.ox.ac.uk/~bioimaging/bitm/instructions_and_information/EM/neg_stain.pdf 8) Staining, retrieved from: en.wikipedia.org/wiki/staining 13) G.Hariprasad, Bacterial Staining, 2011, retrieved from: www.slideshare.net/doctorrao/bacterial-staining.methods.