Lab Report Enzyme Experiment

Hypothesis:
If pH is increased or decreased past the enzyme's optimum pH, the number of products produced by the enzyme will decrease because the enzyme will become denatured.

Variables:
The Independent variable is the pH of the environment. The uncertainty of pH is ± 1. pH is a unitless value.
The Dependent variable is the number of products produced. The uncertainty of this this measurement is ± 1 product.
In order for this experiment to be controlled, many variable were identified and held constant. If these variables were not to be held constant than the findings of this experiment would be meaningless because there would be no way of desiring if the independent variable was the cause of the changes which were recorded In this experiment,
The average number of products produced was calculated by adding the sum of the number of products produced in each trial and dividing by the number of total trials. This value was recorded on the far right hand column of the data table.
The procedure in steps 4-12, was repeated for each of the other thirteen pH values.

Experimental Set-up

Data:
The Effect of pH on the number of products produced

Qualitative Data:
When the pH of the environment was 1, an average of 5 products were produced. As this pH value increased to 2, an average of 8 products were produced. The large quantity of products was produced when the pH was equal to four. When the pH value increased to five, the average amount of product which was produced decreased to 9. As the pH values continued to increase, the number of products produced decreased even further. When the pH was equal to 8, zero products were produced. All of the pH values which were higher than 8, yielded the same result; zero products produced.

Statistical Analysis:
Calculation 1: Average Number of Products Produced
Average = term 1+term 2...+term n n
Average = 3+5+5+3+7+7 6
Average = 30 6
Average =
The enzyme produced the largest number of products when the pH was four. When the environment became more basic, the enzyme produced fewer products. Only nine products were produced when the pH of the environment was 5. Furthermore, with a pH of seven, even fewer products were produced. For all pH values which were greater than seven, zero products were produced. Additionally as the pH of the environment decreased to values less than four, fewer and fewer products were produced. When the pH was three, only nine products were produced and when the pH was one only five products were produced. All of the data was not reliable because there was an extremely wide range of data which was collected. When the pH of five was tested, the maximum amount of products produced was thirteen while the lowest value was five. Furthermore, the control variable were the pH was neutral signifies that the acidity or basicity of an enzyme's environment impacts its productivity. Even though the enzyme did not have its optimum pH at seven, this signifies that the enzyme likely functions in an acidic environment, because the optimum pH for the enzyme was four. An enzyme is a protein whose structure is dependent on hydrogen bonding, when these hydrogen bonds are interfered with, the structure of the enzyme will change. An enzyme’s structure is essential to its function in