Laboratory Experiments
Name: Lawrence D. Mandras Date: August 17, 2013 Rating:_____
Yr. & Course: BSED-IV
Laboratory Experiment no. 2
The Living Plant Cell
Objectives:
* To study the different types of plant cells. * To observe plant cells as to their shapes, structures and function.
Materials:
Microscope cover slip glass slide water onion
Iodine soln. hydrilla salt tomato razor blade
Medicinal dropper epidermal tissue of rhoeo-discolor
Procedure:
1. Onion Cell 1. Prepare an onion bulb 2. Place a drop of water at the center of the slide. 3. Pell off a thin layer from the inner surface of onion bulb [Allium cepa]. 4. Put a cover slip on the specimen and focus under LPO then draw 3 to 4 cells and label parts. Then slowly shift to HPO and label parts that are clearly magnified. Remove the water from the by pressing one side of cover slip. 5. Apply a drop of iodine solution [IKI] on the opposite side of the cover slip. Wait for minute and wipe off excess IKI solution. Observe under HPO and answer the question.
2. Hydrilla or Water plant 1. Take a leaf from a shoot of water plant which has been exposed to sunlight. 2. Mount the upper surface of the leaf. Focus the LPO up and down, you can see two layers of cells. 3. Under the HPO observe the streaming protoplast and note the direction of its movement [cyclosis]. Draw 3 to 4 cells and label the parts. Focus on the tooth-like cell along the margin of the leaf under the HPO. Inside the cell is a jelly-like material called cytoplasm. The clear area, the vacuole is fluid, the cell sap. 3. Epidermal Tissues 1. Prepare the specimen w/c is the rhoeo discolor. 2. Mount both upper and lower epidermis of the leaf. 3. Examine the lower epidermis under LPO and HPO. 4. Tomato: Locate cells that are bounded by a single wall called primary wall. 5. Locate the middle lamella, the intercellular layer
Yr. & Course: BSED-IV
Laboratory Experiment no. 2
The Living Plant Cell
Objectives:
* To study the different types of plant cells. * To observe plant cells as to their shapes, structures and function.
Materials:
Microscope cover slip glass slide water onion
Iodine soln. hydrilla salt tomato razor blade
Medicinal dropper epidermal tissue of rhoeo-discolor
Procedure:
1. Onion Cell 1. Prepare an onion bulb 2. Place a drop of water at the center of the slide. 3. Pell off a thin layer from the inner surface of onion bulb [Allium cepa]. 4. Put a cover slip on the specimen and focus under LPO then draw 3 to 4 cells and label parts. Then slowly shift to HPO and label parts that are clearly magnified. Remove the water from the by pressing one side of cover slip. 5. Apply a drop of iodine solution [IKI] on the opposite side of the cover slip. Wait for minute and wipe off excess IKI solution. Observe under HPO and answer the question.
2. Hydrilla or Water plant 1. Take a leaf from a shoot of water plant which has been exposed to sunlight. 2. Mount the upper surface of the leaf. Focus the LPO up and down, you can see two layers of cells. 3. Under the HPO observe the streaming protoplast and note the direction of its movement [cyclosis]. Draw 3 to 4 cells and label the parts. Focus on the tooth-like cell along the margin of the leaf under the HPO. Inside the cell is a jelly-like material called cytoplasm. The clear area, the vacuole is fluid, the cell sap. 3. Epidermal Tissues 1. Prepare the specimen w/c is the rhoeo discolor. 2. Mount both upper and lower epidermis of the leaf. 3. Examine the lower epidermis under LPO and HPO. 4. Tomato: Locate cells that are bounded by a single wall called primary wall. 5. Locate the middle lamella, the intercellular layer