LSM3232 Medicinal Microbiology Lab Report
INTRODUCTION
Many infectious diseases caused by infectious agents, including bacteria, fungi, viruses and parasites, have plagued human existence CITATION Kon97 \l 1033 (Koneman, 1997). Thus the identification of bacteria becomes all the more important in the search for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars, various other tests need to be done to differentiate and confirm their identity.
RESULTS
Bacteriology – When grown on MacConkey agar, A had abundant growth of pink punctiform colonies that are circular and moist. Microbes A are gram-negative cocci. B had moderate growth of yellow punctiform colonies that are circular and moist. Microbes B cells are gram-positive cocci. C had abundant 2mm growth of pink colonies that are circular and have a mucoid consistency. Microbes C are gram-negative rods bacilli. D had abundant growth of 2mm yellow colonies that are circular and have a mucoid consistency. Microbes D are gram-negative bacilli that gave off a fishy smell. Swarming was observed on plate D. E has an abundant growth of gram-negative, 2mm circular blue-green colonies with diffused green pigments. E gave off a pungent odour and have a mucoid consistency. No growth was observed for F.
When grown on blood agar under aerobic conditions, A had abundant growth of 1mm white, circular, moist colonies that exhibited gamma hemolysis. B had moderate growth of 1mm white, circular, moist colonies that exhibited gamma hemolysis. C had abundant growth of 2mm white, circular, mucoid colonies that exhibited alpha hemolysis. D had abundant growth of 2mm white, circular, mucoid colonies that exhibited alpha hemolysis. Swarming was observed in plate D and colonies gave off a fishy smell. E has an abundant growth of 6mm blue-green colonies of indistinguishable shape with diffused green pigments. E exhibited beta hemolysis.
Many infectious diseases caused by infectious agents, including bacteria, fungi, viruses and parasites, have plagued human existence CITATION Kon97 \l 1033 (Koneman, 1997). Thus the identification of bacteria becomes all the more important in the search for medicines and cure. Identification of bacteria is a multistep process because while some preliminary guesses can be made from the morphology of microbes on various differential agars, various other tests need to be done to differentiate and confirm their identity.
RESULTS
Bacteriology – When grown on MacConkey agar, A had abundant growth of pink punctiform colonies that are circular and moist. Microbes A are gram-negative cocci. B had moderate growth of yellow punctiform colonies that are circular and moist. Microbes B cells are gram-positive cocci. C had abundant 2mm growth of pink colonies that are circular and have a mucoid consistency. Microbes C are gram-negative rods bacilli. D had abundant growth of 2mm yellow colonies that are circular and have a mucoid consistency. Microbes D are gram-negative bacilli that gave off a fishy smell. Swarming was observed on plate D. E has an abundant growth of gram-negative, 2mm circular blue-green colonies with diffused green pigments. E gave off a pungent odour and have a mucoid consistency. No growth was observed for F.
When grown on blood agar under aerobic conditions, A had abundant growth of 1mm white, circular, moist colonies that exhibited gamma hemolysis. B had moderate growth of 1mm white, circular, moist colonies that exhibited gamma hemolysis. C had abundant growth of 2mm white, circular, mucoid colonies that exhibited alpha hemolysis. D had abundant growth of 2mm white, circular, mucoid colonies that exhibited alpha hemolysis. Swarming was observed in plate D and colonies gave off a fishy smell. E has an abundant growth of 6mm blue-green colonies of indistinguishable shape with diffused green pigments. E exhibited beta hemolysis.
References: Koneman, E. W. (1997). Koneman 's Color Atlas and Textbook of Diagnostic Microbiology. USA: Lippincott Williams & Wilkins. Madigan, et. al (2014). Brock Biology of Microorganisms (14th ed., p. 78). Boston: Pearson. Mahon, C., Lehman, D., & Manuselis, G. (2011). Textbook of Diagnostic Microbiology. Missouri: Elsevier. Tille, P. M. (2014). Bailey & Scott 's Diagnostic Microbiology. Missouri: Elsevier.