M3: Glucose Solution Concentration (%)
Task 1c – M3:
Glucose Solution Concentration (%) Absorbance (Arbitrary Units)
1 1.61
0.1 0.32
0.01 0.23
0.001 0.22
How to use a Colorimeter:
• A Colorimeter measures the absorbance of particular wavelengths of light by a specific solution.
• Firstly to use the Colorimeter you must switch it on 5 minutes before use to allow it to stabilize. Next select the most appropriate filter for the analysis and then anter it into the light channel or the selector depending on the type of Colorimeter you are using to measure your sample, then select a blank solution to also put into the Colorimeter ensuring it is turned towards the light channel e.g. water and ensure that the machine is set to zero this allows the Colorimeter to calibrate for efficient use. Finally insert the solution being tested and read the absorbance figures shown on the screen of the solution and record appropriately.
How to use a Pipette:
• A pipette is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid. Pipettes come in several designs for various purposes with differing levels of accuracy and precision. …show more content…
Next wash the pipette with distilled water to ensure the risk of contamination is reduced as they may have potential residue from previous experiments. Then place the pipette a beaker of distilled water, holding the bulb attached to the top and selectively releasing to fill the pipette with the water, then removing the bulb and placing your finger over the end and rotating to ensure the distilled water coats the inside of the pipette, once the pipette is sufficiently decontaminated lift your finger of the end of the pipette and allow the water to run into an empty beaker. Once this is complete carry out the same procedure for the solution being
Glucose Solution Concentration (%) Absorbance (Arbitrary Units)
1 1.61
0.1 0.32
0.01 0.23
0.001 0.22
How to use a Colorimeter:
• A Colorimeter measures the absorbance of particular wavelengths of light by a specific solution.
• Firstly to use the Colorimeter you must switch it on 5 minutes before use to allow it to stabilize. Next select the most appropriate filter for the analysis and then anter it into the light channel or the selector depending on the type of Colorimeter you are using to measure your sample, then select a blank solution to also put into the Colorimeter ensuring it is turned towards the light channel e.g. water and ensure that the machine is set to zero this allows the Colorimeter to calibrate for efficient use. Finally insert the solution being tested and read the absorbance figures shown on the screen of the solution and record appropriately.
How to use a Pipette:
• A pipette is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid. Pipettes come in several designs for various purposes with differing levels of accuracy and precision. …show more content…
Next wash the pipette with distilled water to ensure the risk of contamination is reduced as they may have potential residue from previous experiments. Then place the pipette a beaker of distilled water, holding the bulb attached to the top and selectively releasing to fill the pipette with the water, then removing the bulb and placing your finger over the end and rotating to ensure the distilled water coats the inside of the pipette, once the pipette is sufficiently decontaminated lift your finger of the end of the pipette and allow the water to run into an empty beaker. Once this is complete carry out the same procedure for the solution being