Method Synthesis Lab Report

2.3. Method Development
2.3.1. Preparation of Stock Solution
100 mg of the synthesized compound was made up to 100 ml into a 100ml standard flask with ethanol to give a solution of 1000 μg/ml.
2.3.2. Determination of λ max
1 ml of stock solution was pipetted out was made up to 10 ml into a 10ml standard flask with ethanol to obtain strength 100 μg/ml and scanned at 200-400nm using a UV spectrophotome-ter (Deepak V Bageshwar et al., 2010).
2.3.3. Preparation of Standard Calibration Curve
Aliquots of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1 ml were pipetted out into 10 ml vol-umetric flask and made up with ethanol. The linear calibration curve was plotted within the range from 1-10 µg/ml concentration analyzed by UV spectrophotometer by plotting absorb-ance against concentration.
Structural Elucidation
The synthesized compound was structurally characterized by IR, NMR and mass spectropho-tometer. An IR spectral study was done with Shimadzu Corporation, Japan. An NMR spec-tral study was done with Bruker Fourier- NMR spectrometer. Mass spectra of the samples were recorded on a Waters e 2695-Waters 3100 instruments with ESI-PMT arrangement as the mode of ionization and type of detector respectively (Manasa K et al.,