Microbiology Lab Chap 1
Observing Bacteria and Blood
Cynthia Alonzo, M.S. Version 42-0249-00-01
Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Exercise 1: Viewing Prepared Slides
Questions
A. Identify the following parts of the microscope and describe the function of each.
A=Ocular Lens- Magnifies the image as it brings it to your eye (Alonzo p52).
B= Body Tube- Connects to the arm of the microscope with a gear that allows you to focus the image to focus the image when changing lenses or observers and moving the lenses away from the stage when changing specimens (Alonzo p53).
C= Condenser- A Lens system that aligns and focuses the light from the lamp onto the specimen (Alonzo p53)
D= Objective Lens- Gathers light from the specimen (Alonzo p53).
E= Stage- where the specimen is placed (Alonzo p52).
F= Diaphragm in the light path to alter the amount of light that reaches the condenser (Alonzo p52)
G= Lamp- Produces Light (Alonzo p52).
H= Course Focus Knob- Brings the object into the focal plane of the objective lens (Alonzo p53).
I= Fine focus Knob- Makes fine adjustments to focus the image better (Alonzo p53).
J= Arm- The curved portion that holds the microscopes optical parts in place and keeps them aligned (Alonzo p53).
K= Clip- Holds the specimen secure on the stage (Alonzo p52).
L= Base –Supports the weight of the microscope (Alonzo p53).
Define the following microscopy terms:
Cynthia Alonzo, M.S. Version 42-0249-00-01
Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Exercise 1: Viewing Prepared Slides
Questions
A. Identify the following parts of the microscope and describe the function of each.
A=Ocular Lens- Magnifies the image as it brings it to your eye (Alonzo p52).
B= Body Tube- Connects to the arm of the microscope with a gear that allows you to focus the image to focus the image when changing lenses or observers and moving the lenses away from the stage when changing specimens (Alonzo p53).
C= Condenser- A Lens system that aligns and focuses the light from the lamp onto the specimen (Alonzo p53)
D= Objective Lens- Gathers light from the specimen (Alonzo p53).
E= Stage- where the specimen is placed (Alonzo p52).
F= Diaphragm in the light path to alter the amount of light that reaches the condenser (Alonzo p52)
G= Lamp- Produces Light (Alonzo p52).
H= Course Focus Knob- Brings the object into the focal plane of the objective lens (Alonzo p53).
I= Fine focus Knob- Makes fine adjustments to focus the image better (Alonzo p53).
J= Arm- The curved portion that holds the microscopes optical parts in place and keeps them aligned (Alonzo p53).
K= Clip- Holds the specimen secure on the stage (Alonzo p52).
L= Base –Supports the weight of the microscope (Alonzo p53).
Define the following microscopy terms:
References: Alonzo, Cynthia. (2008) Microbiology: A Laboratory Manual of Small-Scale Experiments For The Independent Study of Microbiology. Englewood, CO: Hands-On Labs, Inc.