Minimum Inhibitory Concentration Lab Report

Minimum Inhibitory Concentration (MIC) is a way to measure the sensitivity of microbes to antibiotics or antifungals (antimicrobials). Organisms are tested for growth in various concentrations of antibiotic. This allows microbiologists to see which concentration (MIC) of antimicrobial will be effective against the pathogen. It also used for determining treatment for patients suffering from infections such as pneumonia, meningitis, endocarditis or osteomyelitis or managing the treatment of high-risk patients such as those suffering from cystic fibrosis or immunocompromised individuals. MIC methods are varies such as disc tests that give qualitative result, dilution tests which testing the ability of microorganisms to produce visible growth on several agar plates. It containing dilutions of the culture and antimicrobial agent.
Firstly, a LB broth of Escherichia Coli culture was adjusted the turbidity until it is similar to the 0.5 McFarland standard. McFarland standard are used as reference to adjust the turbidity of bacterial suspension so that the number of bacteria will be within the given range. Hence, the culture turbidity achieved had an absorbance of 0.132 range at wavelength 540nm. Then, the E.coli was inoculated on the nutrient agar plate by swabbing using cotton bud in all direction to ensure there are no gaps are left in the deposited inoculum. The M.I.C.E strip we used contained different concentration gradient of antibiotic Gentamycin. The strip was removed from sachet using sterile forceps on the black region of M.I.C.E strip and the end with lowest concentration was applied onto the plate first. Then, carefully we rolled the strip onto the agar surface to ensure good contact along the entire of strip. The plate was leaved a while so the strip not fallen after inverted. The plate was incubated