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Neighbor-Vocration Experiment: Phylogenetic Analysis Of Chitinolytic Activity

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Neighbor-Vocration Experiment: Phylogenetic Analysis Of Chitinolytic Activity
The spot inoculation method was followed for antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared, sterilized at 121ºC, poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus, was spread on the same plate and reincubated at 27º C for 3 days.
RESULT
Analysis of Chitinolytic Activity
After the incubation the bacterial strain which showed a highest zone of clearance in the colloidal chitin medium indicated that it had the ability to …show more content…
Sequence was deposited in GenBank under accession number (JQ735954).
Phylogenetic Analysis
The evolutionary history was inferred in the Neighbor-Joining method and optimal tree with the sum of branch length = 0.04468882 is shown Figure 2. The isolated strain TS33 was found closely related to Paenibacillus elgii strain NBRC 100335.
Chitinase Assay
The chitinase enzyme of Paenibacillus elgii TS33 showed 163U/ml of chitinolytic activity.
Antifungal Activity of Paenibacillus elgii TS33
The antifungal activity was performed against Fusarium solani, Aspergillus parasiticus and Aspergillus fumigates in which Paenibacillus elgii TS33 showed the maximum zone of inhibition against Fusarium solani which was 17mm followed by Aspergillus parasiticus (15mm) and Aspergillus fumigates (14mm) respectively.

DISCUSSION
Chitinases play an imperative role in the breakdown of chitin and potentially in the utilization of chitin as a renewable resource. Screening is one of the most efficient and successful ways of searching for new or suitable microbial enzymes 16. The influence of the carbon sources on chitinase production demonstrated that monosaccharides inhibited chitinase biosynthesis. Recently, interest in
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