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Study Guide for the BME5 Midterm

The midterm will cover information from lectures 1-4. Subject areas that are fair game for the test are listed below. I may not use every single item in the exam. Note that the level of detail required is that which was covered in lecture. Only information covered in lecture will be on the tests (includes info in figures and tables).

Lecture 1
Processes that utilize recombinant DNA technology (and molecular processes that don’t)
Basic science; what it is, how it is performed and why it is important in biotechnology
Bioinformatics defined
Be able to name some non-life science disciplines important in use of biotechnology
Compare and contrast alcohol and lactate (lactic acid) fermentation
Molecular Pharming defined
Animal Biotech for producing products and for research
Bioremediation basics and phytoremediation defined.
Types of aquatic biotechnology
Regulatory Biotech: 3 agencies and their roles
HGP: what it was and when it was completed
SNPs defined and how they can be useful in medicine.
Pharmacogenomics/pharmacogenetics defined.
Gene Therapy defined, basics on how it is performed and current status.
Compare Embryonic and Adult (somatic) stem cells (just the basics)
ELSI: what it stands for and what it focuses on (be able to describe one area of focus)
Know what GINA is and what protections it provides.

Lecture 2
Cells: Prok v Euk, components and organelles (know those in the outline)
Griffith’s experiment; understand what this identified and how it was performed
Approach taken by Avery et al., what they showed and how
Nucleotide structure
Nucleic acids (DNA and RNA know how they differ) chromatin vs chromosomes chromosome structure: telomeres, centromeres, chromatids
Human Genome: all info in the lecture outline under this heading.
Central Dogma of Molecular Genetics
Cell Cycle stages and what occurs in each
Replication; location, timing, semiconservative nature, mechanism
Mitosis and

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